Philippine E-Journals

Identification of Microbial Isolates for Antagonistic Effects against Phytophthora palmivora

Wenceslao Palma Gil

Discipline: Medicine

 

Abstract:

This study was conducted to identify microbial isolates (MI), among the different colonies on the Potato-Dextrose-Agar (PDA) medium that could be used as a biological control (biocon) for Phytophthora palmivora. The whole process was done by preparing 60 petri plates divided into five groups of 12 plates each. The first group of 12 isolates were planted with P. palmivora as control, while the remaining four groups were planted with different microbial isolates. All the plates were observed at pre-arranged intervals. The entire process was repeated three times to establish the reliability of the results. The percentage of inhibition of the radial growth (% IRG) of the different microbial isolates against the causative agent were measured and recorded. The data gathered were statistically tested using one-way ANOVA. Having computed for the significance of each of the isolates, the following conclusions are derived: Microbial Isolates 1 and 2 do not have significant differences between their growth and that of the P. palmivora. Microbial Isolates 3 and 4 present significant differences. Microbial Isolate 3 is identified as Ceratocystis paradoxa, while Microbial Isolate 4 is Trichoderma. Both isolates are fungi. The researcher recommends that this study be used as a baseline idea for further researches to look for more and better means to treat plant diseases by the use of biological control method.