(ASCs) can still be viable before transplantation when these are cultured in vitro under hypoxic preconditioning (3% O2 concentration). Samples were divided into two groups, one under hypoxic preconditioning and the other in hyperoxia (20% O2 concentration). Observations were made through flow cytometry, immunofluorescence and immunocytochemical analyses. Flow cytometric analysis showed that in r-ASCs hypoxic culture, the levels of CD90+, CD44+ and CD45- were unaltered, but changed under hyperoxic culture, indicated by the down-regulation of CD90+ and CD44+, and up-regulation of CD45-. Meanwhile, immunocytochemical and immunofluorescence analysis showed that under hypoxic preconditioning, r-ASCs culture expressed quiescent cells with p63 as marker, but the latter remained unexpressed in hyperoxic culture. In conclusion, hypoxic preconditioning with 3% O2 concentration supported r-ASCs in sustaining viability before transplantation in rabbit.