Home⇛The Philippine Journal of Biochemistry and Molecular Biology (PJBMB)⇛vol. 8 no. 1 (1988)

Enzymatic Activities Associated with Opacification Factors from Pseudomonas aeruginosa

Marilou Gagalac-nicolas | Salvador Salceda | Lourdes Cruz

Discipline: Biological and Sport Sciences

 

Abstract:

Extracellular proteinconcentrate from the culture filtrate of Pseudomonas aeruginosa was fractionated in Cellex D, an anion exchanger. Fouropacification factors were resolved. Determinationof enzymaticactivity andlyophilization studies showed that a protease may have contributed to effecting corneal injury, but for P. aeruginosa grown in 10% peptone broth, the primary enzyme involved in the opacification process was a hyaluronidase-like enzyme.



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