Home⇛The Philippine Journal of Biochemistry and Molecular Biology (PJBMB)⇛vol. 3 no. 1 and 2 (1980)

In Vivo Assay of Leaf Nitrate Reductase Activity in Coconut (Cocos nucifera) Seedlings

Hari Hartiko | Ernesto J. del Rosario

 

Abstract:

The in vivo nitrate reductase assay of leaf discs of five-to twelve-month old coconut seedlings was optimized in terms of the concentrations of nitrate and dodecyl sulfate, pH, temperature, incubation time, presoaking treatment and other experimental conditions. Enzyme activity was maximal in 0.08% sodium dodecyl sulfate and 0.1 M sodium nitrate at pH 8.0 and was increased by presoaking the leaf discs in 0.1 M phosphate (ph 8.0), especially in the presence of 0.08% sodium dodecyl sulfate.



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