Incorporation of 14c from (u-14c) Acetate Into Amino Acids of Precipitable Proteins
Relicardo M. Coloso | Lourdes Cruz
Abstract:
Ion-exchange chromatography in conjunction with radioisotope labelling techniques were used to investigate the incorporation o..f 14c from (U-14c) -acetate into amino acids of precipitable proteins in P. monodon juveniles. Highest 14c radioactivity was found in the acidic amino acids, aspartic acid and glutamic acid. Little but significant radioactivity was observed in alanine, cysteine, glycine, proline and serine. Amino acids which incorporated little or no 14c were arginine, histidine, isoleucine, lysine, methionine, phenylalanine, threonine, tyrosine and valine. It appears that P. monodon juveniles could not synthesize these amino acids from acetate. The essential nature of these amino acids in P. monodon is discussed.
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