Isolation And Physicochemical Characterization Of Seed Proteins In Rice Bean (Vigna Umbellata (Thumb.) Owhi And Ohashi)
Myrna S. Rodriguez | Evelyn Mae T. Mendoza
Abstract:
Globulins comprised the largest fraction in rice bean seed proteins and accounted for 50 to 60%
of total proteins. with albumin at 17 to 26%, prolamin at 1.5 to 1.8% and glutelin al 3.2 LO 4.2%.
Globulins separated into three peaks with molecular weighlS of approximately > 700,000, for the
first peak, 175,000 and 18,000 for accession (ace) 28; 138,000 and 7,000 for ace 46 and 60,000 and
3,000 for ace PROC l. Albumins exhibited 3 major peaks and a minor one. The major peak had
a molecular weight >210,000. The other peaks had: 75,000, 3,900 and 1,700 for ace 28; 144,000,
14,800 and 2,000 for ace 26 and 56,000, 7,800 and 3,400 for Ace PROC 1. Both albumins and
globulins exhibited heterogeneity showing 7 to 10 bands on polycrylamide gel electrophoresis.
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