This study was conducted to describe the ultrasonographicappearance, measurements and echo mean values of the ovaries and uterus of water buffaloes at various physiological states. Twenty-four apparently healthy, purebred and crossbred dairy water buffaloes were grouped as in estrus (7), non-pregnant (11) and pregnant (6). B-mode ultrasonography of the ovaries and uterus was conducted transrectally using an ultrasound machine equipped with a 5.0 MHz linear array scanner. The ovaries in all animals appeared as non-homogenous hypoechoic ovoid structures with anechoic follicles. Ovarian mean measurements in animals in estrus tended to be higher than in pregnant and non-pregnant animals. The uterus of non- pregnant and pregnant animals appeared as thick hyperechoic band while the lumen was homogenously hypoechoic. The uterine wall of animals in estrus appeared hyperechoic and thin while the lumen was nonhomogenous with distinct anechoic areas. Animals in estrus had significantly thinner left horn wall compared to non-pregnant and pregnant animals. The echo mean values of the ovaries and uterus of buffaloes in estrus were significantly lower than in non-pregnant and pregnant buffaloes. The study suggests that echo mean values of the ovaries and uterus may be considered in determination of the appropriate time for artificial insemination in water buffaloes.