In the Philippines, ready-to-lay (RTL) hens are often sold to poultry layer farms as new stocks or replacement. These stocks are transported from the supplier farm via trucks which can last from hours to days. This study aimed to determine the eﬀect of various transportation durations using a mechanical vibration simulator on the productivity and selected haematological and biochemical indices of laying hens. Results revealed that increasing vibration (stressor) duration exposure elevated the total white blood cell count, and heterophil-to-lymphocyte ratio (H/ L) of the hens, an indicator of stress levels. Even with the shortest vibration exposure of 2 hours, H/ L values were still elevated until the 2nd week. Blood glucose level was found signifcantly diﬀerent between treatments, but with no correlation with feed intake. No significant diﬀerence was observed on plasma protein values and egg production among treatments. The stressor did not aﬀect egg production but had an eﬀect on the animal’s feed intake.