Philippine E-Journals

ERYTHROCYTIC OXIDATIVE STRESS INDICES AND CLINICO-BIOCHEMICAL ALTERATIONS IN GASTROENTERITIS IN DOGS WITH VARIED CLINICAL SCORES

Amiya Kumar Rautray | Ramesh Chandra Patra | Ghanashyam S. Parida | Kautuk Kumar Sardar | Niranjan Panda

 

Abstract:

The erythrocytic lipid peroxidation and antioxidant enzymes were assessed in 106 dogs with diarrhea and/ or vomition and 21 apparently healthy dogs. The total clinical score (CS) was calculated based on fecal consistency, depression and dehydration. Twenty-fve of 106 affected dogs were moderately affected (CS = 4 to 6), and 81 were severely affected (CS = 7 to 9). The dogs’ erythrocyte oxidative index (lipid peroxides level, LPO) and antioxidant enzyme activities (superoxide dismutase, SOD and catalase, CAT) were measured with respect to clinical score, ±blood in stool, breed and sex of the dog. The severely affected dogs had signifcantly (p<0.05) higher LPO (7.16 ±0.20 vs 4.94 ±0.17 nmol of MDA/mg of Hb) and CAT activities (0.33 ±0.01 vs. 0.21 ±0.01 units/mg of Hb) as compared to control dogs with clinical score 0. Dogs with bloody stool (n=39) with mean clinical score of 8.56 ±0.08 had signifcantly lower SOD activity (1.56 ±0.06 vs 1.93 ±0.09 units/ mg of Hb) as compared to dogs without blood in stool (mean CS 6.73 ±0.17). However, the level of LPO, and activities of SOD and CAT were signifcantly higher than healthy dogs (CS 0). It is concluded that increasing CS was associated with increased level of LPO along with alteration in activities of the antioxidant enzymes such as SOD and CAT.