Fifteen adult female 5-9 years old non-descript dogs were used to determine the ultrasonographic features of experimentally-induced corneal ulcer treated with normal saline solution, tobramycin and autologous serum (Groups A, B and C, respectively) at day 7, 14 and 21. An ultrasound machine equipped with a 7.5 MHz linear array scanner with an aid of a modified stand-off pad was utilized in this study. Upon ultrasound examination, the hyperechoic cornea, iris sphincter, anechoic anterior and posterior chamber were seen. The lens and vitreal chamber and the retina with other choroidal layers which appeared as a mesh of hyperechoic and hypoechoic structures were successfully visualized. Corneal ulceration appeared as hypoechoic with hyperechoic structure. The mean±SD central corneal thickness was 589.87±0.252 μm. Groups B and C had significantly greater decrease in corneal ulcer depth and gap at day 21 than Group A. Echo mean analysis revealed increasing echogenicity as the healing process progressed throughout the observation period and could be attributed to fibroblastic proliferation. The results show that ultrasound can be used to monitor and evaluate the healing of corneal ulcers.