Though the frequency of occurrence of the dog erythrocyte antigen (DEA) 1 system is well known in many countries, little information is available for dogs in India. The study was conducted to determine the prevalence of the DEA 1 blood group (1.1 and 1.2 subgroups) utilizing 100 1-9 years old dogs of both sexes and various breeds. Blood collected from the cephalic or saphenous vein was immediately subjected to standard DEA 1 blood typing procedure using a commercially available KABB Dog Blood Typing Kit. The prevalence of DEA 1 blood type system was observed to be 78%, of which 52% and 26% of the animals were positive for DEA 1.1 and DEA 1.2, respectively. Considerable variation was observed in DEA 1.1 and 1.2 prevalence among different breeds with Pug and German Shepherd showing the highest prevalence for DEA 1.1 and DEA 1.2, respectively. In conclusion, this study demonstrated general frequencies of DEA 1, DEA 1.1, DEA 1.2 blood types as 78%, 52% and 26%, respectively in different breeds of dogs in the Punjab State of India.