The study aimed to analyze the chemical constituents, antibacterial properties, and cytotoxic activities of Pycnoporus coccineus. Fresh thalli of P. coccineus were prepared as sample for the proximate chemical composition analysis which was performed by gravimetric methods. Ethanolic extract of the comminuted and pulverized thalli of P. coccineus was prepared by maceration technique and the crude extract was used for phytochemical screening through thin layer chromatography in order to determine the secondary metabolites. More crude extract was used for the detection of antibacterial property through the minimum inhibitory concentration (MIC) assay against three different species of bacteria, Escherichia coli, Salmonella typhi and Staphylococcus aureus, and for the detection of cytotoxic activity through the brine shrimp lethality (BSLA) assay against brine shrimp. The thallus of P. coccineus was found to have high protein content and total ash which would indicate mineral contents. The secondary metabolites detected were of steroids, sugars, anthraquinones, coumarines, anthrones, tannins, phenol, flavonoids, and alkaloids. The toxicity level was found out to be relatively active with LC50 of 488.28µg/mL but the antibacterial activity is only partially active against S. typhi.