The emergence of different diseases may pose health risk to all people. Nonetheless, Philippines is blessed with natural resources which can be utilized for treating diseases. One of them is a Sandoricum koetjape (Santol) in which the antibacterial action of fresh and dried leaf extracts against bacterial strains such as Staphylococcus aureus, Staphylococcus epidermidis, Salmonella typhimurium, Methicillinresistant Staphylococcus aureus (MRSA) ATCC 43300 and isolate, Bacillus cereus, Escherichia coli 0157:H7, Listeria monocytogenes, and Pseudomonas aeruginosa were the focus of this study. Kirby-Bauer method was performed for antibacterial action having Vancomycin and Azithromycin as positive controls. T-test analyses revealed no significant difference in the efficacy between positive control and fresh leaf extracts against S. aureus, S. epidermidis, S. typhimurium, B. cereus, E. coli 0157:H7, MRSA ATCC and isolate. However, there is a significant difference against L. monocytogenes, and P. aeruginosa. In the case of dried leaf extract, there is no significant difference in the zone of inhibition exhibited by S. epidermidis, S. typhimurium, B. cereus, E. coli 0157:H7, MRSA ATCC 43300 and isolate. But, there is a significant difference in S. aureus, L. monocytogenes, and P. aeruginosa. The findings reveal that the positive control is more potent antibacterial drug against the microorganisms than the leaf extracts of Santol.