Plant is widely used as a source of medicines by some Mindanaoan tribes. The researchers aimed to study five folkloric plants used by five different tribes to treat different illnesses and sickness. These plants were collected and selected based on the interview with the traditional healers of Manobo tribe of Kimagting, Kalilangan Bukidnon, Matigsalog tribe of Kimanait, Kalilangan Bukidnon, Talaandig tribe of Pangantucan Bukidnon, Maranao tribe of Kalilangan Bukidnon and the Higanon tribe Ulayan, Kalilangan Bukidnon. The plants were identified as Amor-seco (Bidens pilosa), Anuang (Kyllinga monocephala), Bilabila (Eleusine indica), Bugang (Pennisetum purpureum), and Gemilina (Gmelina arborea). The said plants were tested on its alkaloids, cardenolides and bufadienolides, anthraquinones, flavonoids, saponins and tannins. Field test was done in Bukidnon and remaining tests in University of the Immaculate Conception. Results showedabsence of alkaloid while saponins were only present in K. monocephala and P. purpureum. Steroids were detected to all plants except E. indica and K. monocephala. While tannins were also detected in all plants except for K. monocephala and B. pilosa. Flavonoids were shown to be positive in B. pilosa and G. arborea. All were negative in anthraquinones.