Diabetes mellitus is a chronic disease that can be severely complicated when the patient incur foot ulcer. In fact, development of vascular complications including failure of wound healing process has increased disease mortality over the years. There are different brands of antimicrobial cleansing agents dispensed in Davao City, many are expensive. The most common wound cleanser is Hydrogen Peroxide. This study aimed to perform a microbiological assay of Normal Saline Solution combined with Zonrox as a possible diabetic foot wound cleanser compared with Hydrogen Peroxide as positive control. The study utilized microbiological disk diffusion technique to determine the sensitivity of diabetic wound bacterial isolate by measuring the zones of inhibition (mm). The isolates were swabbed out of the wounds of three diabetic patients and cultured aseptically. Based on the reference sensitivity value of ≥16mm zone of inhibition, all bacterial isolates were are found to be sensitive to both the experimental and control agents. Interestingly, using ANOVA, there is a significant difference (p<0.05) in the zones of inhibition (mm) between Hydrogen Peroxide and Normal Saline Solution combined with Zonrox in favor for the experimental agent. Hence, the Normal Saline Solution combined with Zonrox exhibited greater zones of inhibition, and may be further tested as an alternative diabetic foot wound cleanser.