Bacteria can be found everywhere. Though most are relatively harmless, many microorganisms are harmful and can cause death. Bacteria can thrive in restrooms as moisture and organic wastes support their growth. Urinary tract and gastrointestinal infections are the most commonly encountered health problems acquired from restrooms. Thus, this study attempted to determine the presence of bacteria in two randomly selected male and female restrooms in restaurant bars in Prime Square, F. Torres Street, Davao City. By swabbing, Gram staining and biochemical test methods, most of the isolated microorganisms were identified to be Neisseria species, followed by Staphylococcus aureus, S. epidermidis, E. coli, Bacillus species., Enterobacter, Klebsiella, Enterococcus, E. saprophyticus, Micrococcus and Salmonella. Most of the bacteria isolated were normal flora known to cause opportunistic infections of urinary tract, skin and gastrointestinal tract, and some are serious pathogens. These results show that public restrooms lack proper maintenance and that users must practice personal hygiene after using public restrooms.