Blood testing is one of the most common tests performed in a clinical laboratory. The venipuncture method, if not done properly, might lead to any of the known complications like hematoma, needle phobia, anxiety and fainting. This led the researchers to embark on the study using other body fluids specifically, saliva and urine, as substitute of blood for the determination of cholesterol, glucose and uric acid levels in the body. The study was done by obtaining blood, urine, and saliva samples from fifteen patients with pathologic conditions, namely: Hypercholesterolemia, Diabetes Mellitus, and Gouty Arthritis. Following standard protocol for spectrophotometry, urine and saliva samples were tested for cholesterol, glucose, and uric acid. The tests were done three times with no specific time interval. The mean values obtained from saliva and urine was compared to that in the blood. The correlation between the results from saliva and blood, and urine and blood were determined with the use of Spearman – rho coefficient. The study showed that there is no significant relationship between the levels of cholesterol, glucose and uric acid in saliva and urine with that in the blood. However, the results revealed a significant relationship between the level of glucose in saliva and blood.