The purpose of this study was to investigate the effect of sapogenin crude extract of sambiloto (Andrographis paniculata) on creatinine and blood urea nitrogen (BUN) levels and on nephrotoxicity in gentamicin-induced rats. Fifty apparently healthy rats (Rattus norvegicus) were divided equally into five groups: 1) K-, given sapogenin crude extract of sambiloto (SCES) carrier; 2) P0, given SCES carrier and gentamicin; 3) P1, given SCES at 3.78 mg/200 g body weight and gentamicin; 4) P2, given SCES at 7.56 mg/200 g body weight and gentamicin; 5) P3, given SCES at 11.34 mg/200 g body weight and gentamicin. The rats were observed for 14 days. Blood samples were collected, the animals were euthanized and the kidneys and examined histopathologically. Parameters measured were creatinine and BUN levels and histopathological changes in renal structures of gentamicin-induced rats. Data were analyzed with one-way ANOVA and least significant difference test. Results suggest that sapogenin crude extract from sambiloto can reduce creatinine and BUN levels and prevent nephrotoxicity in gentamicin-induced nephrotoxicity in rats.