The purpose of this study was to test whether human chorionic gonadotropin (hCG) from urine of pregnant women can be used for in vitro maturation of Madura cattle oocytes. Urine samples were collected from 50 healthy pregnant women gestating for 1.5 to 3.5 months. Molecular weights of hCG were 37 kDa and 22 kDa. HCG levels measured via ELISA had 10 times the deposition, with an average of 27,333 mIU/l at 1.5 months and 105,667 mIU/l at 3.5 months, respectively. T-test showed no significant difference in oocyte maturation rate between the control group with patent hCG and hCG from pregnant women at P>0.05. This study illustrates that hCG from pregnant women at 1.5 to 3.5 months can aid in in vitro maturationof Madura cattle oocytes.