Philippine E-Journals

Phytochemical and antibacterial screening of the pseudostem of Musa (BBB group) ‘Saba’ against canine skin pathogens

Beulah T Grevialde | Anne Julian S Ladiana

Discipline: Public Health

 

Abstract:

This study was conducted to determine the presence of phytochemicals and the antibacterial activity of the pseudostem of Musa (BBB Group) ‘Saba’ against canine skin pathogens. The fresh and rotten pseudostem methanolic extracts were tested for the presence of different phytochemicals such as flavonoids, alkaloids, saponins, tannins and terpenoids through test tube reaction and thin layer chromatography. Antibacterial activity of the two pseudostem extracts were tested through agar well diffusion method against the test microorganisms including Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and the isolated pathogen Staphylococcus pseudintermedius from the canine model organism. Both the pseudostem extracts were mixed with petroleum jelly to prepare the ointment, which was tested on the canine for 10 days. The results reveal that the fresh and rotten extracts are potential sources of alkaloids, saponins, tannins and terpenoids. For the antibacterial activity, the rotten pseudostem extract showed partially active inhibition against S. areus and S. pseudintermedius only. The fresh pseudostem extract showed inactive inhibition against all the bacterial strains used. The wound healing activity of the rotten pseudostem ointment was observed effective as early as the 5th day of application, while the fresh pseudostem ointment was observed to take effect at the 10th day of application.