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HomeIAMURE International Journal of Ecology and Conservationvol. 30 no. 1 (2020)

Microbicidal Activity of Tinospora rumphii boerl, Anamirta cocculus, and Andrographis paniculata Species on Gram Positive and Gram-Negative Organisms

Nero M. Paderes | Maria Digna T Bose



Richly endowed, the province of Abra in the Philippines is abundant with a wide variety of traditional medicinal plants. Moreover, these are the general sources of medicine for the local people. Microbicidal analysis of threeindigenous plants was done using the Kirby-Bauer disc diffusion method. Te technique involves the soaking of filter paper discs having the same sizes into the different plant extract formulated at 75%, 50% and 25% concentrations. Sub-cultures of gram-positive and gram-negative representative organisms were obtained from the Department of Science and Technology Microbiology Laboratory located in Taguig City Metro Manila. Likewise, all bacterial cultures were checked for purity and maintained in nutrient agar slants. Additionally, the organisms tested were Staphylococcus aureus (G+), and Escherichia coli (G-).Te result shows that Tinospora rumphii boerl produced a strong microbicidal activity towards gram positive organisms (S. aureus) in all concentrations. Consequently, it has no inhibitory activity on gram negative organisms (E. coli). Anamirta cocculus, on the other hand, has been observed to affect both S. aureus and E. coli with a weak to moderate inhibitory activity from the different extract concentrations. Lastly, Andrographis paniculata, on the other hand, is moderately active against E. coli and weak towards S. aureus.