This study aimed to test the antibacterial eﬀect of termite mushroom (Termitomyces clypeatus) and false truffle (Scleroderma polyrhizum) against Staphylococcus aureusand Escherichia coli”. Specifically, it aimed: (1) to determine the zone of inhibition produced by the diﬀerent extracts of termite mushroom and false truffle against S.aureus and E.coli at 75%, 50%, and 25% concentration; (2) to determine if there is a significant diﬀerence between the eﬀects of the diﬀerent extracts of termite mushroom and false truffle against S. aureus and E. coli at 75%, 50%, and 25% concentration; (3) to determine which of the three concentrations of the extracts of termite mushroom and false truffle has the best antibacterial eﬀect against S. aureus and E. coli. Based on the findings, at 50% concentration both termite mushroom and false truffle extracts exhibited antibacterial activity against Staphylococcus aureus with a mean zone of inhibition of 19.34 mm and 19.97 mm respectively. Among the diﬀerent concentrations of extracts, 50% concentration has the highest mean followed by 75% concentration and 25% concentration. Analysis of Variance (ANOVA) revealed a highly significant diﬀerence existed among diﬀerent treatments, which means that diﬀerent concentrations have diﬀerent inhibitory eﬀects. The researchers recommend the following: phytochemical analysis of termite mushroom and false truffle must be conducted; other bacterial strains should be tested; since E. coli and S. aureus are sensitive at 50% concentration of the termite mushroom and false truffle extracts, it is recommended that it could be used as an antibacterial treatment; and further studies on antibacterial eﬀect of other indigenous mushrooms should be conducted.