The study was conducted to enhance the Technological Pedagogical Content Knowledge (TPACK) of selected public school science teachers in Quezon province through development of a training program. The mixed method of research design was utilized in the study wherein quantitative data and qualitative data were triangulated. The respondents were thirty (30) science teachers from Division of Lucena City, Division of Tayabas City and Division of Quezon. The weighted mean, t-test for significance and one-way ANOVA were used as statistical treatment of the data. Based on the findings, it was concluded that the respondents have sufficient knowledge in each domain of Technological Pedagogical Content Knowledge (TPACK). However, aiming to elevate higher the TPACK of science teachers, an enhancement training program was developed. Implemented to the respondents, it is valid for use and it can enhance the science teachers’ TPACK level regardless of their age, sex, and teaching experience. It was recommended that science teachers may consider using the TPACK framework in order to develop their knowledge level and to enhance the teaching and learning processes. School administrators may also adapt the TPACK training program as part of their faculty development program.