Philippine E-Journals

The Antibacterial Efficacy of Crude Vitis vinifera (Grape) Seed Extract Against Staphylococcus aureus and Escherichia coli

Ruth Esther Marie C Antenor | Anne Penelope C Azucena | Reyn Vianca A Cabral

 

Abstract:

Antimicrobial is coined from the Greek words "anti" meaning against, "mikros" referring to small, and "bios" meaning life. Antimicrobials are agents that act against all types of microorganisms. Often, plant extract is one of the main ingredients in making these antimicrobials. In this study, the researchers used ethanolic crude extract of the Vitis vinifera (grape) seed to determine its efficacy as an antibacterial agent. The extracts were tested against Staphylococcus aureus and Escherichia coli using cylinder plate assay. Zones of inhibition produced by the fruit extracts were measured, then statistical treatment to determine its significance was employed. There was a considerable difference between the represented value of the positive and the known concentration. The positive control, having a mean value of 50ppm, is significantly the highest concentration for the given extract. However, after testing the antibacterial property of the grape seed extract, the result showed that on the given concentration, there was no zone of inhibition, which means it is not effective against Staphylococcus aureus and Escherichia coli. Therefore, Vitis vinifera (grape) seed ethanolic crude extract cannot be used as an antibacterial agent against Staphylococcus aureus and Escherichia coli.