The study aimed to determine the growth and yield of oyster mushroom (Pleurotus sp) affected by different substrates and booster application. The three levels of boosters ( Control, Commercial Booster, and Rice Wash) and six levels of substrates (Control, 100% Rice Straw, 100% Sawdust, 50% Rice Straw+50% Sawdust, 50% Sawdust+50% Sugarcane Bagasse, and 50% Rice Straw+50 % Sugarcane Bagasse) served as treatments. Data were analyzed using the Two Way Analysis of Variance. Tukey’s Honest Significant Difference Test and Least Significant Difference Test were used to compare treatment means. Results showed that the number of days from inoculation to primordial initiation and harvesting of oyster mushroom was significantly the shortest with Rice Wash as a booster. Moreover, it also produced the longest and most number of stalks, while Commercial Booster generated a significantly bigger stalk diameter. On the other hand, oyster mushroom spawn inoculated in 50 % RS+50 % SB significantly produced the least number of days from inoculation to full colonization, primordia initiation, and harvesting. Weight, stalk diameter, and length, were significantly the highest in oyster mushrooms grown in 50% RS+50 %SD. Rice Wash’s use as a booster and 50% RS + 50% SD as substrates are recommended for mushroom production. Different proportions of the substrates and boosters evaluated must also be tried. Also, other available indigenous substrates and possible boosters in the locality should be explored.