Philippine E-Journals

Cryopreservation of boar (Sus domesticus) semen using lactose egg yolk (LEY) cooling and freezing extender

Manuel P. Granadozin Jr. | Lucia M. Lucia M. Rigos | Lerma C. Ocampo | Flocerfida P. Aquino

 

Abstract:

The study was conducted primarily to verify the usefulness of the recommended Boar Semen Cryopreservation protocol based on the USDA procedure at the National Animal Germplasm Program with specific objectives to cryopreserve boar semen using the Lactose Egg Yolk (LEY) freezing procedure and to compare the two extenders (Androhep and Beltsville Thawing Solution) used for the transport of ejaculated boar semen from a locally raised boar studs of known fertility. Seven trials (n=7) were done with Beltsville Thawing Solution (BTS) and Androhep extenders. Following semen collection, evaluation for macroscopic and microscopic characteristics was done. The t-test for independent samples was used to determine the differences between the two treatments (p<0.05). Results showed numerical difference in the mean values for initial volume of sperm (2.97±0.85 and 3.53±0.89), pH level (7.21±0.57 and 7.42±0.19), sperm concentration (2.35±0.57 and 2.96± 1.11), percentage motility prior to addition of LEY (67.86±4.88 and 69.29±6.07), percentage sperm liveability (71.45±7.44 and 71.69± 5.63) and percentage normal sperm (83.57±3.82 and 82.43± 4.68) for Androhep and BTS respectively. However, both treatment means did not differ significantly. Also, the result showed a numerical difference in post-thaw motility (24.29±10.18 and 25.71±11.70), post-thaw percentage sperm liveability (28.11±8.52 and 34.20±10.50) and percentage normal sperm (21.86±2.95 and 19.36±2.86) for Androhep and BTS respectively but did not differ significantly in T-test. The USDA cryopreservation protocol using LEY cooling and freezing extender which was optimized by the study proved to be feasible under the Philippine laboratory condition as comparable results were achieved.