Philippine E-Journals

Minimum Inhibitory Concentrations of Aminoglycoside and B-Lactam Antibiotics Against Nosocomial Pseudomonas Aeruginosa Isolates from the Philippine General Hospital and their Transconjugants

Esperanza C. Cabrera

Discipline: Microbiology

 

Abstract:

The levels of resistance to newer aminoglycosUk and Blactam antibiotics of nosocomia/Pseudomonas aeruginosa isolates and their transconjugants were determined by testing for the minimum inhibitory concentrations (MI C's) of the antibiotics against rhe isolates. The MI C's of the test antibiotics against the microorganisms were at kast 100% higher than those of the concentrations used in the modified KirbyBaun disc - agar diffosion ~thod of antibiotic sensitivity testing. This high level of resistance suggests that the resistance are plasmid-~diated. The nosocomial P. aeruginosa isolates and their respective transconjugants were also shown to have different levels of resistance to the antibiotics. Pmu/omonas aeruginosa has emerged as a major pathogen among nosocomial or hospital-acquired infections (Legakis et al. 1987; Giamarellou et al. 1986; Farmer et al. 1982). Increased use of antibiotics over the years has led to the introduction of positive selective pressure for antibiotic resistant microbial strains to survive, rapidly increase in number, be disseminated in the environment and in the population