Philippine E-Journals

Antiproliferative Activity and Apoptotic Effect of Premna odorata Blanco Leaves against Hepatocellular Carcinoma Cell Line (HEPG2)

Jed Pauline C. Alvarado | Farizah Mezer Afdo Anami | Marinell Leamina T. Andres | Krisella Anne R. Bayudan | Brian A. Brinces | Jonas Erik DL Cabrera | Mihran Roger Gian F. Dela Cruz | Pamela E. Delos Reyes | Benedict C. Reginald | Mark Arnold Q. Gatus | Jomer Patrick L. Heyres | Austin Ralph M. Vargas | Delia R. Lim

 

Abstract:

Background: This study aimed to determine the antiproliferative activity and apoptotic effect of Premna odorata Blanco against hepatocellular carcinoma cell line (HepG2). Methodology: In this experimental study, MTT assay was used to assess the antiproliferative activity of the extract. HepG2 cells were treated with three groups: extract, doxorubicin (positive control) and DMSO (negative control). Eight two-fold dilutions of the extract and doxorubicin were used while DMSO had a concentration of 0.1% (v/v). The absorbance was then read at 570 nm at 3 trials and the inhibitory concentration (IC50) and percent inhibition were computed using GraphPad Prism 6. Caspase-3 assay was used to evaluate the apoptotic effect of the extract. Similar concentrations of the three groups were used and the induction concentration (150) and percent inhibition were computed with the same software. Representative photomicrographs of cells before and after treatment were captured using inverted microscope at 400x. Results: Results showed that extract exhibited concentration-dependent inhibition of proliferation with IC50 of 64.78 ug/ml. It also revealed a concentration-dependent induction of apoptosis with I50 of 123.2 ug/ml. All concentrations of the extract were comparable to doxorubicin at certain concentrations (p < 0.05). Conclusion: These findings suggest that P. odorata Blanco is a potential chemotherapeutic plant that warrants further study and evaluation.