Antiproliferative Activity and Apoptotic Effect of Premna odorata Blanco Leaves against Hepatocellular Carcinoma Cell Line (HEPG2)

Delia  R. Lim,; Jed Pauline  C. Alvarado,; Farizah Mezer Afdo  Anami,; Marinell Leamina  T. Andres,; Krisella Anne  R. Bayudan,; Brian  A. Brinces,; Jonas Erik  D. Cabrera,; Mihran Roger Gian  F. Dela Cruz,; Pamela  E. Delos Reyes,; Benedict  C. Reginald,; Mark Arnold  Q. Gatus,; Jomer Patrick  L. Heyres,; Austin Ralph  M. Vargas,

Antiproliferative Activity and Apoptotic Effect of Premna odorata Blanco Leaves against Hepatocellular Carcinoma Cell Line (HEPG2)

Abstract:

Background: This study aimed to determine the antiproliferative activity and apoptotic effect of Premna odorata Blanco against hepatocellular carcinoma cell line (HepG2). Methodology: In this experimental study, MTT assay was used to assess the antiproliferative activity of the extract. HepG2 cells were treated with three groups: extract, doxorubicin (positive control) and DMSO (negative control). Eight two-fold dilutions of the extract and doxorubicin were used while DMSO had a concentration of 0.1% (v/v). The absorbance was then read at 570 nm at 3 trials and the inhibitory concentration (IC50) and percent inhibition were computed using GraphPad Prism 6. Caspase-3 assay was used to evaluate the apoptotic effect of the extract. Similar concentrations of the three groups were used and the induction concentration (150) and percent inhibition were computed with the same software. Representative photomicrographs of cells before and after treatment were captured using inverted microscope at 400x. Results: Results showed that extract exhibited concentration-dependent inhibition of proliferation with IC50 of 64.78 ug/ml. It also revealed a concentration-dependent induction of apoptosis with I50 of 123.2 ug/ml. All concentrations of the extract were comparable to doxorubicin at certain concentrations (p < 0.05). Conclusion: These findings suggest that P. odorata Blanco is a potential chemotherapeutic plant that warrants further study and evaluation.

References:

WHO.int [Internet]. 2012. Available from: https://www.who.int
Leading Cause of Mortality. (n.d.). [cited 2018, Apr 8]. Available from http://www.doh.gov.ph/Statistics/Leading-Causes-of-Mortality
Waghray A, Mural AR, Menon K. Hepatocellular carcinoma: From diagnosis to treatment. World Journal of Hepatology. 2015; 7(8), 1020. doi:10.4254/wjh.v7.i8.1020
Greenwell M., Rahman P. Medicinal Plants: Their Use in Anticancer Treatment. International Journal of Pharmaceutical Sciences and Research. 2015 [cited 2018Apr 8].
antengco OA, Jacinto SD. Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines. Asian Pacific Journal of Tropical Biomedicine. 2015;5(12), 1037-1041. doi:10.1016/j.apjtb.2015.09.011
Pinzon LC, Uy MM, Sze K, Wang M, Chu I. Isolation and characterization of antimicrobial, anti-inflammatory and chemopreventive flavones from Premna odorata Blanco. Journal of Medicinal Plants Research. 2011; 5(13). Retrieved April 9, 2018.
Elmaidomy AH, Mohyeldin MM, Ibrahim MM, Hassan HM, Amin E, Rateb ME, et al. Acylated Iridoids and Rhamnopyranoses from Premna odorata (Lamiaceae) as Novel Mesenchymal-Epithelial Transition Factor Receptor Inhibitors for the Control of Breast Cancer. Phytotherapy Research. 2017; 31(10), 1546-1556. doi:10.1002/ptr.5882
Riss TL, Moravec RA, Niles AL, Duellman S, Benink HA, Worzella, TJ, Minor L. Cell Viability Assays. In Assay Guidance Manual. 2016.
ATCC. Hep G2 [HEPG2] [Brochure]. Author. [cited 2018 Apr 8]. Available from https://www.atcc.org/~/ps/HB-8065.ashx
Jokhadze M, Eristavi L, Kutchukhidze J, Chariot A, Angenot L, Tits M, et al. In vitro cytotoxicity of some medicinal plants from Georgian Amaryllidaceae. Phytotherapy Research. 2007; 21(7): 622–624.
Selassie C, Kapur S, Verma, R, Rosario M. Cellular Apoptosis and Cytotoxicity of Phenolic Compounds: A Quantitative Structure−Activity Relationship Study. Journal of medicinal chemistry. 2005; 48. 7234-42. 10.1021/jm050567w.
Chichioco-Hernandez CL, Paguigan ND. Antimutagenic potential and phytochemical analysis of selected Philippine plants. Pharmacognosy Magazine. 2009; Retrieved April 8, 2018.