Home⇛Health Sciences Journal⇛vol. 7 no. 2 (2018)

Effectiveness of immature Mangifera indica Linn (mango) fruit in reducing the Ascaris lumbricoides infection among children: a non-inferiority randomized controlled trial

Arianna Julia S. Enriquez | Grachella Jana Beatriz M. Erlano | John Ruben A. Esperanza | Michael Kevin H. Espino | Jan Paola B. Frayna | Anne Christine E. Gagui | Gerald M. Gaitos | Raquelynne M. Galicia | Joseph R. Gallardo | James Rainier M. Garcia | Ma. Cristina Z. Garcia | Jose Ronilo G. Juangco

 

Abstract:

Introduction : This study aimed to compare the effectiveness of immature Magnifera indica L. (mango) fruit with albendazole in reducing Ascaris lumbricoides infection among children. Methods : Children aged 2 to 14 years were enrolled in a randomized, controlled, non-inferiority trial. Participants were randomly allocated to receive 250 mL immature mango fruit puree daily for 3 days or one dose of albendazole 400 mg tablet. Egg reduction rates and cure rates were computed and compared. Adverse effects were monitored during and after administration of treatment. Results : There was a statistically significant decrease between the pre- and post-treatment EPG of those who took immature mango fruit (p < 0.001) and those who took albendazole (p < 0.001). There was a higher ERR and CR for the albendazole group, but the difference was not significant (p = 0.472, p = 785, respectively). Risk analysis of reduction in intensity showed mango is non-inferior to albendazole (RR = 0.80, 95% CI 0.67, 0.97; p = 0.026). Risk analysis of cure showed mango is noninferior to albendazole in both PP (RR = 0.92, 95% CI 0.68, 1.25; p = 0.607) and ITT (RR=0.79, 95% CI 0.58, 1.08; p = 0.139). Conclusion : Immature Mangifera indica Linn is non-inferior to albendazole in terms of effectiveness in the reduction of ascariasis infection.



References:

  1. WHO. What are intestinal worms (soil transmitted helminthiasis)? World Health Organization. Available from: http://www.who.int/intestinal_worms/disease/en/. [Cited 2016 Sep 1].
  2. Jia TW, Melville S, Utzinger J, King CH, Zhou XN. Soil transmitted helminth reinfection after drug treatment: a systematic review and meta-analysis. PLOS. 2012 May.
  3. Scolari C, Torti C, Beltrame A, Mateelli A, Castelli F, Gulletta M, Ribas M, Morana S, Urbani C. Prevalence and distribution of soil-transmitted helminth (STH) infections in urban and indigenous school children in Ortigueira, State of Parania, Brasil: implications for control. Trop Med Int Health 2000; 5(4): 302-7.
  4. Gyorkos TW, Maheu-Giroux M, Blouin B, Casapia M. Impact of health education on soil-transmitted helminth infections in school children of the Peruvian Amazon: a cluster-randomized controlled trial. PLoS Negl Trop Dis 2013; 7(9): e2397.
  5. Sabin Vaccine Institute. Ascariasis. Available from: http://www.globalnetwork.org/ascariasis. [Cited 2016 Sep 1].
  6. Belizario V, De Leon W, Wambangco M, Esparar D. Baseline assessment of intestinal parasitism in selected public elementary schools in Luzon, Visayas and Mindanao. Acta Med Philipp 2002; 39(2): 11-21.
  7. Mohan C, Saxena N, Fozdar BI. Activity of indigenously known angiospermic plants against common GI parasites of livestock. World J Pharm Pharmaceut Sci 2015; 4(9): 1652-67. Available from: https://www.wjpps.com/download/article/1441367821.pdf
  8. James CE, Davey MW. Increased expression of ABC transport proteins is associated with ivermectin resistance in the model nematode Caenorhabditis elegans. Int J Parasitol 2009; 39: 213-20.
  9. James CE, Davey MW. A rapid colorimetric assay for the quantification of the viability of free living larvae of nematodes in vitro. Parasitol Res 2007; 101(4): 975-80.
  10. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12(4): 564-82.
  11. Martinez-Micaelo N, González-Abuín N, Ardèvol A, Pinent M, Blay MT. Procyanidins and inflammation: molecular targets and health implications. Biofactors 2012; 38(4): 257-65.
  12. El-Sherbini G, Osman, S. Antihelminthic activity of unripe Mangifera indica L (mango) against Strongyloides stercoralis. Int J Curr Microbiol Appl Sci 2013; 2(5): 401- 9.
  13. Nery PS, Nogueira FA, Oliveira NJF, Martins ER, Duarte ER. Efficacy of extracts of immature mango on ovine gastrointestinal nematodes. Parasitol Res 2012; 111(6): 2467-71. doi: 10.1007/s00436-012-3017-4
  14. Terrill T, Waghorn GC, Wooley DJ, McNabb WC, Barry TN. Assay and digestion of 14C-labelled condensed tannins in the gastrointestinal tract of sheep. Br J Nutr 1994; 72(3): 467-77.
  15. Masibo M, He Q. Major mango polyphenols and their potential significance to human health. Compr Rev Food Sci Food Saf 2008; 7(4): 309-19. doi: 10.1111/j.1541-4337.2008.00047.x
  16. Schieber A, Ullrich W, Carle R. Characterization of polyphenols in mango puree concentrate by HPLC with diode array and mass spectrometric detection. Innov Food Sci Emerg Technol 2000; 1(2): 161-6.
  17. Iqbal Z, Sarwar M, Jabbar A, et al. Direct and indirect anthelmintic effects of condensed tannins in sheep. Vet parasitol 2007; 144(1-2): 125-31. Available from: https://www.researchgate.net/publication6699277_Direct_and_indirect_anthelmintic_effects_of_condensed_tannins_
  18. Thompson DP, Geary T. Biochemistry and molecular biology of parasites. 1995. The structure and function of helminth surfaces, Available from: https://www.researchgate.net/publication/279607588_The_Structure_and_Function_of_Helminth_Surfaces
  19. Page AP, Johnstone II. The cuticle. In: Kramer JM, Moerman DG, editors. Wormbook. The C. elegans Research Community; 2007. Available from: http://www.wormbook.org/chapters/www_cuticle/cuticle.pdf
  20. Leles D, Gardner SL, Reinhard K, Iñiguez A, Araujo A. Are Ascaris lumbricoides and Ascaris suum a single species? Parasites & Vectors 2012; 5(1): 1.
  21. Shao CC, Xu MJ, Alasaad S, et al. Comparative analysis of microRNA profiles between adult Ascaris lumbricoides and Ascaris suum. BMC Vet Res 2014; 10(1): 1.
  22. Niezen JH, Waghor n GC, Graham T, Carter JL, Leathwick DM. The effect of diet fed to lambs on subsequent development of Trichostrongylus colubriformis larvae in vitro and on pasture. Vet Parasitol 2002; 105: 269-83
  23. Ferreira D, Brandt EV, Coetzee J, Malan E. Condensed tannins. In: Progress in the chemistry of organic natural products 1999; 21-67. doi: 10.1007/978-3-7091-6366-5_2
  24. Renco M, Sasanelli N, Maistrello L; Davis LM, editor. Nematodes: comparative genomics, disease management and ecological importance. 1st ed. New York: NOVA Science: 2014.
  25. Garcia D, Escalante M, Delgado R, Ubeira FM, Leiro J. Anthelminthic and antiallergic activities of Mangifera indica L. stem bark components vimang and mangiferin. Phytother Res 2003; 17: 1203-08.
  26. Kawasaki I, Jeong M-H, Oh B-K, Shim Y-H. Apigenininhibits larval growth of Caenorhabditis elegans through DAF-16 activation. FEBS Lett 2010; 584: 3587-91.
  27. Wiria AE, Hamid F, Wammes LJ, et al. The effect of three-monthly albendazole treatment on malarial parasitemia and allergy: a household-based cluster- randomized, double blind, placebo-controlled trial. PLoS ONE 2013; 8(3): e57899. doi: 10.1371/journal.pone.0057899
  28. Yap P, Du Z-W, Wu F-W, et al. Rapid re-infection with soil-transmitted helminths after triple-dose albendazole treatment of school-aged children in Yunnan, People's Republic of China. Am J Trop Med Hyg 2013; 89(1). doi: 10.4269
  29. Jia TW, Melville S, Utzinger J, King CH, Zhou XN. Soil transmitted helminth reinfection after drug treatment: A systematic review and meta-analysis. PLOS. 2012 May 8
  30. Upatham E, Vivavant V, Brockelman W, Kurathong S, Lee P, Chindaphol U. Prevalence, incidence, intensity and associated morbidity of intestinal helminths in south Thailand. Int J Parasitol 1989; 19(2): 217-28.

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