Synergistic activity of Malunggay (Moringa oleifera) leaves and Sodium percarbonate (2Na2CO3•3H2O2) as a wastewater deodorizer for pig farms
Stacy Eleoriene T. Arzadon
Abstract:
According to the Agriculture and Consumer Health Department (2017),
odor is associated with the main environmental and societal problems
throughout pig farms affecting 72% of nearby residents and 30% of
workers in the Philippines (Drucker et al., 2007). The synergistic activity
of Malunggay (Moringa oleifera) leaves with Sodium percarbonate
(2Na2CO3•3H2O2) was aimed at deodorizing pig farm wastewaters to
decrease the presence of odorant compounds. Investigations were: I.
Screening for peroxidase, II. Laboratory analyses for Volatile Organic
Compounds (VOCs), Hydrogen sulfide (H2S), and Ammonia (NH3), III.
Perceptual Odor Assessment of Human Panel, IV. Shelf-life
determination. Screening showed that malunggay leaves contain
peroxidase, an enzyme that can convert toxic materials into less harmful
substances (Albuquerque et al., 2019). For laboratory analyses in the
experimental and negative control groups, the Colorimetry for H2S
showed 48 mg/L and <1.0 mg/L respectively; Phenate method for NH3
showed 124 mg/L and 121 mg/L respectively; Gas Chromatography
detected no other VOC except chloroform. 32 µg/L and 17 µg/L were
found, respectively. Using the 9-point Hedonic scale for perceptual odor
assessment of a human panel, results showed that in terms of odor
intensity, there was an 82.76% decrease in the negative control
(Strongest Odor Imaginable) to the experimental (Moderate Odor).
While there was an 80% decrease in unpleasantness from the negative
control (Extremely Unpleasant) to the experimental (Neutral
Pleasantness). After shelf-life determination, the malunggay leaves
powder lasted for 8 weeks. For further validation of results, additional
studies on the synergistic activity of plant peroxidases and peroxides
must be conducted with various methods of application and testing.
References:
- Aarnink, A., Drucker, A., Huynh, T., & Verstegen, M. (2007). Pig production in Cambodia, Laos, Philippines, and Vietnam: A Review. Asian Journal of Agriculture and Development, 4(1), 70-90. https://www.researchgate.net/publication/228669518_Pig_production_in_Cambodia_Laos_Philippines_and_Vietnam_A_Review
- Abiyu, A., Yan, D., Girma, A., Song, X., & Wang, H. (2018). Wastewater treatment potential of Moringa stenopetala over Moringa oleifera as a natural coagulant, antimicrobial agent and heavy metal removals. Cogent Environmental Science Journal, 4(1). https://doi.org/10.1080/23311843.2018.1433507
- Adewaleb, I.O., Agunbiade, O.J., Famutimi, O.G., Kolapo, O.A. (2021). Studies on peroxidase from Moringa oleifera Lam leaves. Heliyon Journal, 7(1). from doi: 10.1016/j.heliyon.2021.e06032
- Agbisit, E., Catelo, M.A., & Dorado, M.A. (2019). Backyard and Commercial Piggeries in the Philippines: Environmental Consequences and Pollution Control Options. Economy and Environment Program for Southeast Asia (EEPSEA)
- Albuquerque, T.L., Rocha, P., & Valderez, M. (2019). Biotechnological Strategies for the Lignin- Based Biorefinery Valorization. Journal in Chemistry, Molecular Sciences and Chemical Engineering. DOI:10.1016/b978-0-12-409547-2.14570-6
- Atkin, A. L., Cai, L, Hales, K.E., Kim, KH., Nickerson, K.W., Parker, D., Patefield, K.D, Spiehs, M.J., & Woodbury, B.L. (2012). Reducing odorous VOC emissions from swine manure using soybean peroxidase and peroxides. Bioresource Technology Journal, 124, 95-104. https://doi.org/10.1016/j.biortech.2012.08.031
- Bruning, K., Koziel, J.A., Maurer, D.L., & Parker, D.B. (2017). Farm-scale testing of soybean peroxidase and calcium peroxide for surficial swine manure treatment and mitigation of odorous VOCs, ammonia and hydrogen sulfide emissions. Atmospheric Environment Journal 166, 467-478. https://doi.org/10.1016/j.atmosenv.2017.07.048
- Bruns, M.A., Govere, E.M., Heinemann, P.H., Kephart, K.B., Tonegawa, M., & Wheeler, E.F. (2005). Deodorization of Swine Manure Using Minced Horseradish Roots and Peroxides. Journal of Agricultural and Food Chemistry 53(12), 4880-4889. doi:10.1021/jf0404290
- Chang, J., Hu, J., Huang, J., Gao, H., Wang, C., Yan, X., Yu, Z., & Zhang, L. (2017). Chemical structures and characteristics of animal manures and composts during composting and assessment of maturity indices. PLoS One Journal 12(6). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5467826/
- Cortus, E. & Stowell, R. (2014). Ambient Air Quality Regulations that Impact Swine Operations. https://porkgateway.org/resource/ambient-air-quality-regulations- that-impact-swine-operations/
- Dingcerlynne, Natavio, & Zotomayor (2013). Calcium Peroxide as an Alternative Source of Hydrogen Peroxide in Fenton’s Process for the Treatment of Public Market Wastewater. https://dche.ceat.uplb.edu.ph/abstract/calcium-peroxide-as-an-alternative-source- of-hydrogen-peroxide-in-fentons-process-for-the-treatment-of-public-market-wastewaster/
- Dong, Z., Liu, X.F., & Yuan, Y.X. (2016). Deodorization of pig manure using lignin peroxidase with different electron acceptors. Journal of the Air & Waste Management Association, 66(4), 420-428. https://doi.org/10.1080/10962247.2016.1144660
- Engel, L., Pannek, C., Tarantik, K.R., & Wöllenstein, J. (2018). Colorimetric Detection of Hydrogen Sulfide in Ambient Air. Proceedings, 2(13), 804. doi:10.3390/proceedings2130804
- Hartman, M.J. & Stohs, S.J. (2015). Review of the Safety and Efficacy of Moringa oleifera. Phytotherapy research journal. 29(6), 796-804 https://doi.org/10.1002/ptr.5325
- Hameed, A., Noreen, R., & Rai, A.K. (2017). Antioxidant potential and biochemical analysis of Moringa oleifera leaves. International Journal of Agriculture and Biology, 19, 941-950. DOI: 10.17957/IJAB/15.0366
- Kalus, K., Koziel, J.A., Maurer, D., Opaliński, O., & Rice, S. (2017). Odor-reducing microbial mineral additive for poultry manure treatment. Frontiers of Environmental Science & Engineering Journal, 11(3). DOI: 10.1007/s11783-017-0928-4
- Karim, R., Khatun, S., & Pervin F. (2012). Purification and characterization of peroxidase from Moringa Oleifera L. Leaves. Bioresources Journal, 7(3), 3237-3251. https://www.researchgate.net/publication/232273338_Purification_and_characterization_of_peroxidase_from_Moringa_Oleifera_L_Leaves
- Kim, K.Y. & Ko, H.J. (2019). Indoor distribution characteristics of airborne bacteria in pig buildings as influenced by season and housing type. Asian-Australasian Journal of Animal Science, 32(5), 742-747. https://doi.org/10.5713/ajas.18.0415
- Kolhe, P.M., Ingle, S.T., & Wagh, N.D. (2015). Degradation of Phenol Containing Wastewater by Advance Catalysis System. Annual Research & Review in Biology Journal, 8(3), 1-15. DOI:10.9734/ARRB/2015/19936
- Kowalski, Z., Makara, A., & Marszalek, M. (2018). Emission of greenhouse gases and odorants from pig slurry - effect on the environment and methods of its reduction. Journal of Society of Ecological Chemistry and Engineering, 25(3), 383-394. DOI: 10.1515/eces-2018-0026
- Palada, M.C. (2017). The moringa industry in the Philippines: status, challenges and opportunities. Acta Horticulturae journal. 1158, 447-454. https://doi.org/10.17660/ActaHortic.2017.1158.52
- Plieth, C. & Vollbehr, S. (2012). Calcium promotes activity and confers heat stability on plant peroxidases. Plant Signaling & Behavior Journal, 7(6), 650-670. doi: 10.4161/psb.20065
- Rezazgui, O. (2015). Towards a bio-inspired photoherbicide: Synthesis and studies of fluorescent tagged or water- soluble porphyrins, from solution to plant cells. https://tel.archives-ouvertes.fr/tel-01326291/document
- Thet, K. & Woo, N. (2020). Gas Chromatography. https://chem.libretexts.org/@go/page/301
- Ucar, D., Zhang, Y., & Angelidaki, I. (2017) An Overview of Electron Acceptors in Microbial Fuel Cells. Frontiers in Microbiology, 8, 643. doi: 10.3389/fmicb.2017.00643
- Więckol-Ryk, A., Białecka, B., & Thomas, M. (2020). Effect of Green Oxidizing Agent on Inhibition of Escherichia coli Present in Livestock Wastes. Water, Air, & Soil Pollution Journal, 231, 466. https://doi.org/10.1007/s11270-020-04824-3
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