HomeUM Research Journalvol. 4 no. 1 (2007)

Targeted Biomolecular-Chemical Screening Approach to Drug Discovery II Fungal Meta-Bolite with Antibiotic and DNA-Binding Properties

Grace L. Patricio | Aniline D. Sacdalan

Discipline: Biochemistry



This study sought to accomplish the following objectives: isolate, purify and screen fungal species from Barangay Santiago dump site which have potential for antimicrobial activity against representatives of gram-positive, gram-negative bacteria, yeast and mold test microorganisms; extract potential antimicrobial compounds from the fungal isolates using ethyl acetate as solvent; determine the relative  Inhibitory Concentration of the crude microbial extract; perform one-dimensional and two-dimensional thin-layer chromatography to determine DNA-binding property of the extract; and identify the fungal isolates up to the genus level. Nine out of 73 fungal isolates produced antibiotics. 27 different compounds were separated from their respective extracts in which only eight showed potential for DNA-binding in the two-dimensional thin layer chromatography. This report will show that bimolecular-chemical screening targeted antibiotics, can be a powerful tool for isolating DNA-binding antibiotics.