This study is a descriptive method of research which is concerned with the relationship of the DOTS client and clinical related factor to the level of effectiveness of the DOTS program. The clinical related factor provides knowledge as to the set-up of the DOTS facility. The researchers used the correlational survey design of research since the purpose of this research study is to correlate the DOTS client and clinical related factor as to the effectiveness of the Directly Observed Treatment Short course (DOTS) for tuberculosis in terms of program administration, information dissemination, diagnostic services and medical supplies, and medical follow up. The researchers used the NTPTB register of each Rural Health Unit for the list of clients who are registered under the Directly Observed Treatment Short course strategy for the fiscal year 2010 as the respondents of this study. There were eighty-two (82) DOTS client identified and diagnosed of having tuberculosis. In the analysis and interpretation of the data, frequency count, mean and percentage are employed particularly in the interpretation of the profile of the respondents and clinical related factor. Coefficient of Correlation and T-test (test of the relationship) is used in determining the influence of the profile of the client and clinical related factor to the effectiveness of the DOTS program. The findings of the study reveal that there is a significant relationship between the profile of the clients, clinical related factors and the level of effectiveness of the Directly Observed Treatment short course program of the RHU.