This study is focused on the development and validation of a concept test in Introductory Physics for Biology students as a diagnostic tool, a misconception test, a formative assessment tool, and a summative test. Its final form is a 50-item multiple-choice concept test in Introductory Physics for Biology 1st term (Mechanics, Fluids & Heat) students wherein each item has four choices. One among the choices serves as the correct answer while the rest are considered distracters. It was originally developed as a 67-item multiple-choice concept test in Introductory Physics for Biology students covering all the topics specified in the syllabus of the course offered at the Philippine Normal University. Classical test item analysis and validation were conducted to establish test validity while item reliability was tested using Cronbach’s Alpha, which was 0.67, and K-R 21, which was 0.70. Analysis of the distracters was done to determine the Physics misconceptions that can be diagnosed by the instrument. The misconception diagnostic capability of the test is a feature, which can be of help to Physics teachers for better concept understanding of students.