The control environment is the foundation for all other components of internal control such as control activities, risk assessment, information and communication, monitoring and helps to provide the necessary structure and discipline, effectively helping to establish the “tone at the top” within an organization. This study was conducted to determine whether the domains of the control environment significantly predict cultural practices in the workplace. Descriptive-correlational method of research was employed in this study. The respondents of this study were the regular and casual employees of the Department of Health Regional Field Office XI, during the Fiscal Year 2011. In this study, the universal sampling was used. The Multiple Regression was used to answer the main problem of the study. It was found out that the level of the control environment and the cultural practices in the workplace in the Department of Health in Region XI is high. The study concluded that the domains of the control environment significantly predict culture in the workplace. The study recommended that the control environment practices must be enhanced and develop to establish strong cultural practices among employees of the entire organization.