Isolation And Identification Of Microorganisms For Polyurethane Degradation
Julie D. Tan | Takuji Ohwada
Abstract:
Plastic wastes decomposition has been a pressing environmental problem
worldwide. In this study, polyurethane (PUR), a thermoset plastic was tested for
biodegradation by polyurethane-degrading microorganisms that were isolated
from a dumpsite at Kamishihoro, Tokachi, Obihiro, Japan. Actinomycetes were the
most abundant microorganisms from soil samples. From the 65 isolated microbial
species, 16 possessed polyurethane-degrading ability. These isolates exhibited
clearing zones on Yeast-extract salts + Agar and gelatin with polyurethane (YES-AG
+ PUR). The PUR-degrading isolates were characterized and identified based on
their DNA sequence patterns. Some isolates belong to the same genus or species.
They were (B03, B04, B07), spp. (B13, B19,
C13a, C15, C17a, C17b), sp. (B20), (B21),
(C10b, C18, C19) and (F04,
F07). sp. coded as C13a, with base sequence homology of 99.7%
with was believed to produce the highest amount of
both exo- and endo-polyurethanases. This was demonstrated by the widest clearing
zones when broth and cell-bound supernatants were inoculated into the YES-AG +
PUR plate.
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ISSN 0116-0710 (Print)