Home⇛Annals of Tropical Research⇛vol. 46 no. 1 (2024)

Isolation, screening and characterization of xylose-fermenting yeasts isolated from sawdust

Adelabu Blessing | Funke Soetan | Michael Taiwo

 

Abstract:

Yeasts have been less frequently reported as xylanase producers compared to bacteria and filamentous fungi. Different cellulosic materials including sawdust are produced on a large scale and these can be used for the production of useful enzymes such as xylanases. Xylanases are a group of enzymes that hydrolyze plant fibers made of xylan hemicellulose. Xylose-fermenting yeasts isolated from soil at a wood processing factory were isolated and qualitatively and quantitatively screened for xylanase production using xylose supplemented medium and congo red as indicator. Xylanase enzyme was produced using different xylose concentrations (0.5%, 1.0%, 1.5% and 2.0%). Pichia chambardii isolate, which was later identified as Wickerhamomyces chambardii by molecular techniques, showed the highest xylanase activity of 199.31U mL . Maximum xylanase activity -1 (275.83U mL ) was achieved at 1.5 %w/v xylose. This -1 study showed that yeasts have a high potential for the production of xylanase enzymes.



References:

  1. Adelabu BA, Kareem OS, Adeogun AI & Wakil SM. 2019. Optimization of cellulase enzyme from sorghum straw by yeasts isolated from plant feeding−termite Zonocerus    variegatus Food and Applied Bioscience Journal.  2019 7(3):81-99
  2. Adelabu BA and Kareem OS. 2022. Cellulase production by immobilized cells of candida tropicalis isolated from grasshopper Zonocerus Variegatus in saw dust and rice husk medium. Polish Journal of Natural Sciences 37(2):187–204
  3. Agbogbo F and Coward-Kelly G. 2008. Cellulosic ethanol production using the naturally occurring xylose-fermenting yeasts, Pichia stipitis Biotechnology Letters 30(9):1515-1524
  4. Amadi OC, Egong JE, Nwagu NT, Okpala G, Onwosi OC, Chukwu CG, Okolo NB, Agub CR  & Moneke NA. 2020. Process optimization for simultaneous production of cellulase, xylanase and ligninase by Saccharomyces cerevisiaesolid SCPW 17 under solid state fermentation using Box-Behnken experimental design. Heliyon 6:34-46
  5. Apiradee S. 2006. Isolation and characterization of thermotolerant yeast for ethanol    production (MS thesis). Suranaree University of Technology (pp98)
  6. Barnett JA and Yarrow D. 2000. Yeasts characteristics and identification (3  edn) rdCambridge University Press (pp11-39)
  7. Bhardwaj N Kumar B & Agarwal K. 2019. Purification and characterization of a thermo-acid/alkali stable xylanases from Aspergillus oryzae LC1 and its application in xylo-oligosaccharides production from lignocellulosic agricultural wastes. Int J Biol Macromol 122:1191-1202
  8. Burlacu A,  Cornea CP  & Isreal-Roming F. 2016. Microbial xylanase: a review. Scientific Bulletin. Series F. Biotechnologies 66:1-26
  9. Giese EC, Dussan KJ, Pierozzi MT, Chandel AK, Pagnocca FC & da Silva SS. 2017. Cellulase production by. Trichosporon laibachiiThe. Orbital: Electronic Journal of Chemistry 9(4):271−278
  10. Gupta J, Rathour R, Singh R & Thakur IS. 2019. Production and characterization of extracellular polymeric substances (EPS) generated by a carbofuran degrading strain Cupriavidus sp. ISTL7. Bioresource Technology 282(pp417–424)
  11. Kahve HI, Akbulut M & Coklar H. 2022. Identification and technological characterization of endogenous yeast isolated from fermented black carrot juice shalgam LWT 154:54 61
  12. Kalim B, Böhringer N, Ali N & Schäberle TF. 2015. Xylanases–from Microbial Origin to Industrial Application. British Biotechnology Journal 7(1):1–20 
  13. Kareem S, Akpan I & Oduntan S. 2009. Cow pea: A novel substrate for solid state production of amylase by Aspergillus oryzae. African Journal Microbiology Research 3(12):974–977
  14. Lee HV, Hamid SB & Zain SK. 2014. Conversion of Lignocellulosic Biomass to Nanocellulose. Structure and Chemical Process 13(4):67–79
  15. Mandel A. 2015. Review on Microbial Xylanases and their Applications. International Journal of Life Sciences 4(3):178–187
  16. Mardawati E, Putri AV, Yuliana T, Rahimah S, Nurjanah S & Hanidah I. 2019. Effects of substrate concentration on bioethanol production from oil palm empty fruit bunches with simultaneous saccharification and fermentation (SSF).  IOP Conferences Series: Earth and Environmental Science 230:1–7
  17. Maryam B, Qadir A, Zameer M, Ahmad S, Nelofer R, Jamil N, ARZOO S & Afzaal R.2018. Production of cellulases by bacillus cellulosilyticus using lignocellulosic material. Polish Journal of Environmental Studies 27(6):2659−2667
  18. Moubasher AH, Abdel−Sater MA & Zeinab S. 2017. Yeasts and filamentous fungi inhabiting guts of three insect species in Assiut, Egypt. Mycosphere 8(9):1297–1316
  19. Papagianni M. and Papamichael ME. 2017. A Pichia anomala Strain (P. Anomala M1) Isolated from Traditional Greek Sausage is an Effective Producer of Extracellular Lipolytic Enzyme in Submerged Fermentation.  Fermentation 3(3):43
  20. Sadaf S and Abdul R. 2013. Isolation and characterization of cellulose degrading Candida tropicalis W2 from environmental samples. Pakistan Journal of Zoology 45(3):809-816
  21. Shankar T, Sankaralingam S, Balachandran C, Chinnathambi A, Nasif O, Ali S, Alharbi O, Park S & Baskar K.  2021. Purification and characterization of carboxymethylcellulase from Bacillus pumilus EWBCM1 isolated from earthworm gut (Eudrilus eugeniae), Journal of King Saud University – Science (33):1-11
  22. Sharma D, Sharma D Mahajan R. 2019. Development of strategy for simultaneous enhanced production of alkaline xylanase-pectinase enzymes by a bacterial isolate in short submerged fermentation cycle. Enzyme and Microbial Technology 122:90-100
  23. Sharma N and Sharma N. 2017. Microbial xylanases and their industrial applications as well as future perspectives: a review. Global Journal of Biology Agriculture and Health Sciences 6(3):(pp5-12)
  24. Taiwo MO, Oyedemi OT, Adelabu BA, Shofunde AA, Ojesola CO & Anthony AC. 2021. Identification and antibiotic susceptibility pattern of Enterobacteriaceae isolated from Gecko (Hemidactylus Frenatus). Journal of Applied Sciences and Environmental Management 25(10):1765-1773

Tools


Copyright © 2025  KITE Digital Educational Solutions |  Exclusively distributed by CE-Logic