HomeIAMURE International Journal of Science and Clinical Laboratoryvol. 5 no. 1 (2014)

DNA-Binding Property of the Most Bioactive Metabolite from the Hexane Extract of the Aerial Part of Hydrocotyle verticillata

Nathalie L. Daminar | Lydia M. Bajo

Discipline: Chemistry



Studies show that some plants contain compounds, the secondary metabolites which are economically important fine chemicals. Many of these chemicals are sources of therapeutic agents like having DNA –Binding ability needed for man’s ailments. In this study, the main interest was to determine whether the most bioactive component of the plant Hydrocotyle verticillata, had DNA-Binding property. Solvent extractions were used to extract the natural components of the aerial part of H. verticillata using n-hexane, ethyl acetate, dichloromethane and water. Bioactivity of the extract was determined using the brine Shrimp Lethality Test; one had the lowest LC50 indicated as the most bioactive extract. Purification of the most bioactive extract was done through a series of Gravity Column Chromatography (GCC) and High Performance Liquid Chromatography Analysis (HPLC). Biomolecular-chemical screening was utilized to evaluate DNA Binding property of the most bioactive component. The results revealed that that n - hexane extract showed to be the most bioactive, and after further purification, four isolates labeled (HF5B1, HF5B2, HF5B3 and HF5B4) were obtained and further yield a subfraction HF5B2a showing its single sharp peak on the HPLC chromatogram with a retention time of 8.135 min. Furthermore, the results show that HF5B2a had Rf2/Rf1 ratio of 0.800 (below 1), thus, implying affinity of the compound towards DNA.