The abundance, availability and affordability of pineapple have triggered the researcher to explore its potential for clinical laboratory applications. This study was conducted to determine the viability of pineapple (Ananas comosus) extract as laboratory anticoagulant and substitute for ethylenediamine tetraacetic acid (EDTA). The data obtained from laboratory standard procedures were subjected to one-way analysis of variance with Tukey’s test. Among the four methods of extraction used, 95% ethanol and Expression gave a considerable yield with respect to volume of the extract produced. With regard to the ability to prevent blood coagulation, extract obtained through Expression with spiral twist obtained positive results comparable for EDTA. In the observation for red blood cell hemolysis, the different volumes of extracts produced results comparable to EDTA. However, with regard to the efficiency in preventing red blood cell crenation and clumping, 300ul extract did not have a significant difference with EDTA; therefore, the results obtained were comparable. Preservation of the staining reactions of the cell structures was also observed. Although significant differences were recorded in each of the volumes used, 300ul produced results that were somehow comparable to EDTA. The efficiency was recorded in the evaluation of the extract’s efficiency in maintaining cell size and shape.