Philippine E-Journals

Single Nucleotide Polymorphisms Promoter Gen Il-10 as Predictor Disease Activities of Systemic Lupus Erythematosus in Javanese Patients

Yuliasih Yuliasih

Discipline: Science

 

Abstract:

Systemic Lupus Erythematosus is a complex disease, signifying that genetic and environmental factors are involved in diseases development. Although the role of genetic factor in many populations had been studied, the genetic factor associated with diseases activity has not been identified yet. This study was performed to determine polymorphisms in the IL-10 gene promoter with diseases activity SLE in Javanese population. This study was performed to analyze the genotype IL-10 gene proximal promoter in SLE patients using a pair of specific primer of the IL-10 gene proximal promoter and sequenced by Applied Biosystem Method. This study enrolled 47 SLE patients (ACR criteria) in active diseases. Disease activity was evaluated using SLAM. The level of IL–10 was measured by ELISA kit. There were three locus SNP at -294 A/G, -296 A/T and -301 A/G of IL- 10 gene proximal promoter, with three variant combination: -294G/-296A/- 301G, -294G/-296T/-301G and -294A/-296A/- 301A. The serum level of IL-10 among three groups was significantly different. The patients with variant -294G/- 296A/-301G had a significantly higher score of disease activity (severe diseases) compared with the other groups of patients. The combination -294A/-296A/- 301A was similar to the sequence IL-10 gene proximal promoter from GenBank NCBI. It is concluded that Interleukin-10 proximal promoter SNP as predictor diseases activity of Systemic Lupus Erythematosus.