Zea mays Aedes aegypti (L.) Pollen as Nutriment to (Diptera: Culicidae) Larvae under Laboratory Conditions
Kristine Mae Y Bentoy | Stephanie L Sayson | Dan Anthony U Bataan | Frances E. Edillo
Abstract:
This study investigated if maize ( Linnaeus) pollen can serve as a Zea mays
nutrient source to Linnaeus larvae, the primary dengue Aedes aegypti
mosquito vector in the Philippines. First instar larvae of were Ae. aegypti
subjected to different treatments of maize pollen reared in mineral water or in
rain water to determine their effects on the larval development into adult
emergence. Results showed that the overall development time, survivorship,
and mean duration of subadult stages differed ( <0.05; univariate ANOVA) but P
not on wing length among the treatments of Larvae fed with maize Ae. aegypti.
pollen and reared in rain water took 7.36 d to emerge into adults and had 65%
survivorship, whereas those reared in mineral water took 7.88 d to become
adults and had 62% survivorship. Larvae in positive control took 6.05 d to
become adults and had 79% survivorship. Mean duration from first to third
instar larval stages differed ( 0.05) among treatments. Post hoc analysis P<
using Scheffe's pairwise comparison test showed that larvae fed with maize
pollen and reared in rain water did not significantly differ ( >0.05) from the P
positive control (fish food) on their development time, survivorship, and mean
duration of subadult stages. These suggest that maize pollen provides
nutrients for larvae similar to the positive control. Carbohydrates Ae. aegypti
and proteins were detected in Molisch, Iodine, and Biuret tests. Results are
relevant for future work in establishing the link between dengue mosquitoes
andmaize plantationsin the Philippines.
References:
- ANDERSON 1.0. J. NATH and E.J. HARNER. 2004. Effects of freeze-preservation on some pollen enzymes: II. Freezing and freeze-drying stresses. Agronomy Journal 64: 420-427.
- BARRERA R., M. AMADOR and G.C. CLARK. 2006. Ecological factors influencing Aedes aegypti (Diptera: Culicidae) productivity in artificial containers in Salinas, Puerto Rico. Journal of Medical Entomology 43: 484-492.
- BOYD C.E. 1999, Water Quality: An Introduction. The Netherlands: Kluwer Academic Publishers Group.
- EDILLO FE., Y.T. TOURE, G.C. LANZARO, D. GUIMOGO and C.E. TAYLOR 2004. Survivorship and distribution of immature Anopheles gambiae s.l. (Diptera: Culicidae) in Banambani Village, Mali. Journal of Medical Entomology 41:333-339.
- EDILLO, F.E., N.B. ROBLE and N.D. OTERO II. 2012. The key breeding sites by pupal survey for dengue mosquito vectors [Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse)] in Guba, Cebu City, Philippines. Southeast Asian Journal of Tropical Medicine and Public Health 43:1-10.
- EDILLO FE. and S. MADARIETA. 2012. Trends of dengue cases and deaths in Cebu Province, Philippines. Dengue Bulletin 36:37-49.
- GLEISER R.M., J. URRUTIA and D.E. GORLA. 2000. Body size variation of the floodwater mosquito Aedes albifasciatus in central Argentina. Medical and Veterinary Entomology 14:38-43.
- GOSS J.A. 1968. Development, physiology and bio-chemistry of corn and wheat pollen. Botanical Review 34:333-358.
- GUHA-SAPIR D. and R. SCHIMMER 2005. Dengue fever: new paradigms for a changing epidemiology. Emerging Themes in Epidemiology 2:1
- HOCHERLN, R SIEDE, LILLIES, H. GATSCHENBERGER and J. TAUTZ 2012. Evaluation of the nutritive value of maize for honey bees. Journal of Insect Physiology 58 (2): 278-285.
- KEBEDE A., J.C. MCCANN, AE. KISZEWSKI and Y YE-EBIYO. 2005. New evidence of the effects of agro-ecologic change on malaria transmission. American Journal of Tropical Medicine and Hygiene 73: 676-680.
- LUNDGREN J.G. and R.N. WIEDENMANN. 2004. Nutritional Suitability of Corn Pollen for the predator Coleomigilla maculata (Coleoptera: Coccinellidae). Journal of Insect Physiology 50:567-575.
- NASCI R.5. 1986. The size of emerging and host-seeking Aedes aegypti and the relation of size to blood-feeding success in the field. Journal of the American Mosquito Control Association 2:61-62
- NELSON KE, CM. WILLIAMS and N.M.H. GRAHAM. 2001. Infectious Disease Epidemiology Theory and Practice. Aspen Publication: Maryland, USA
- RAYNOREGS R.C. OGDEN and J.V. HAYES 1972 Dispersion and deposition of corn pollen from experimental sources. Agronomy Journal 64: 420-427.
- REISEN W.K., K. AZRA and F. MAHMOOD, 1982. Anopheles culicifacies (Diptera: Culicidae): horizontal and vertical estimates of immature development and survivorship in Rural Punjabi Province, Pakistan Journal of Medicul Entomology 19:413-422
- ROULSTON TH. and S.L. BUCHMANN. 2000. A phylogenetic reconsideration of the starch-pollination correlation. Evolutionary Ecology Research 2:627-643.
- SCHORNIG E. 1971. Mosquitoes in Cebu and adjacent areas: An ecological survey. Philippine Scientist 8: 22-32.
- STANDIFER LN. 1966. Some lipid constituents of pollens collected by 309 honeybees. Journal of Apicultural Research S:93-98.
- STANLEY RG. and H.F. LINSKINS. 1974. Pollen: Biology, Biochemistry Management. Springer-Verlag New York.
- (SPSS) Statistical Package for the Social Sciences 2010 Available at: http://www.ihm.com/software/analytics/spss/products/statistics/Accessed January 2010.
- STRICKMAN D. and P. KITTAYAPONG. 2003 Dengue and its vectors in Thailand calculated transmission risk from total pupal counts of Aedes negypti and association of wing-length measurements with aspects of the larval habitat. American Journal of Tropical Medicine and Hygiene 68:209-217.
- UMAR A. and K.N. DON-PEDRO, 2008. The effects of pH on the larvae of Aedes aegypti and Culex quinquefasciatus. International Journal of Pure and Applied Sciences 2(3): 58-62
- (WHO) World Health Organization: Western Pacific Regional Office, 2010 Country Health Information Profiles: Philippines. Available at: http://www.wpro.who.int/NR/rdonlyres/C7648689-4348-442C-A93F 8D83F5F6DD1A/o/30Philippines2009.pdf.
- (WHO) World Health Organization: Western Pacific Regional Office. 2014. Dengue Situation Update 448 (September 23, 2014). Available at: http://www.wpru.who.int/emerging diseases/dengue hiweekly_2.3s ep2014.pdf
- YE-ERIYO Y., A. KISZEWSKI and A. SPIELMAN. 2003. Enhanced development of larval Anopheles arubiensis by proximity to flowering maize (Zeu mays) in turbid water and when crowded. American Journal of Tropical Medicine and Hygiene 68:748-752
- YE-EBIYO Y., R. POLLACK, A. KISZEWSKI and A. SPIELMAN. 2003. A component of maize pollen that stimulates larval mosquitoes (Diptera: Culicidae) to feed and increases toxicity of microbial larvicides, Journal of Medical Entomology 40: 860-864.
- YE-EBIYOY., R. POLLACK and A. SPIELMAN. 2000. Enhanced development in nature of larval Anopheles arabiensis mosquitoes feeding on maize pollen. American Journal of Tropical Medicine and Hygiene 63:90-93,
ISSN 2704-3541 (Online)
ISSN 0116-0710 (Print)