Home⇛Journal of Interdisciplinary Perspectives⇛vol. 3 no. 8 (2025)

Determining the Effect on the Crude Extract of Psophocarpus tetragonolobus (Winged Bean) as Protein Source Replacement for Tryptic Soy Broth

Patricia Angela Bianca T. Tordesillas | Nikson R. Patawaran | Arwin J. Aparente | Lance Edilbert G. Veloso | Jeri Nicole Bautista | Daniel Marl Berches | Eunica Jade Manalang | Angelene Padual | Krizzelle Mae Rañada

Discipline: molecular biology, biophysics and biochemistry

 

Abstract:

This study explores the potential of Psophocarpus tetragonolobus (Winged Bean) crude extract as a protein source replacement for TSB, aiming to address the need for sustainable and cost-effective culture media. This research evaluated the growth of E. coli and S. aureus in modified TSB containing P. tetragonolobus (Winged Bean) crude extract at concentrations of 2%, 5%, and 10%. The TSB standard served as the positive control, and NSS served as the negative control. The media were inoculated with a crude extract prepared from the leaves of P. tetragonolobus (Winged Bean). Growth of bacteria was measured using the bacterial smear count method microscopically in terms of CFU/mL. The results obtained were statistically evaluated using ANOVA and multiple post hoc tests to identify a significant difference across different concentration treatments. The findings of this study indicated that a 5% concentration of P. tetragonolobus (Winged Bean) crude extract was the most viable concentration, with a mean count of 136.67 CFU/mL for E. coli and 145.33 CFU/mL for S. aureus. These values were comparable to those of TSB at 131 CFU/mL and 260.33 CFU/mL for E. coli and S. aureus, respectively. The growth was moderate, at 2%, while it significantly decreased at a 10% concentration, likely due to the inhibitory effects caused by the high solute concentration. These results confirmed that P. tetragonolobus (Winged Bean) crude extract was indeed a feasible source of practical and sustainable protein replacement for TSB.



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