Fungi from Coastal Sediments as Potential Agents in Biodegrading Used Engine Oil

Jayzon  G. Bitacura,; Pamela  P. Abit,; Arvin  C. Balala,

Fungi from Coastal Sediments as Potential Agents in Biodegrading Used Engine Oil

Jayzon G. Bitacura | Arvin C. Balala | Pamela P. Abit

Abstract:

The use of microorganisms to decontaminate the environment loaded with oil pollutants is a beneficial option for bioremediation. This study aimed to isolate, characterize and identify oil-degrading fungi from the coastal sediments of the port area of Ormoc City, Philippines, and to evaluate and compare the oil-biodegrading capabilities of these fungi. Fungal isolates were subjected to an in-vitro biodegradation assay using used engine oil as substrate. Four species of filamentous fungi were isolated and identified down to genus level. The fungal isolates include three Aspergillus spp. and one Penicillium sp.Aspergillus spp.1 & 2 were the most efficient in degrading and utilizing the used engine oil for growth. Aspergillus sp. 1 & 2 and Penicillium sp.had mean pH values of 6.286, 6.136 and 6.32, respectively. Highest mean percent fat loss on the media and highest mean dry weights were exhibited by Aspergillus sp. 2 (mean fat loss = 77.12% and mean dry weight= 0.24g) andAspergillus sp. 1 (mean fat loss = 69.4% and mean dry weight= 0.2 lg). Filamentous fungi from the coastal sediments in the vicinity of Ormoc City port area could therefore be used as potential bioremediation agents in areas contaminated with petroleum oils.

References:

ADEKUNLE, A.A., and O.A. ADEBAMBO. 2007. Petroleum hydrocarbon utilization by fungi isolated from Detarium senegalense (J.F Gmelin) seeds. Journal of American Science. 3(1):69-75.
AL-GHAMDI, A.Y. 2011. Investigating the ability of five fungal species to utilize gasoline as sole carbon source. Egyptian Academic Journal of Biological Science 3(1):7-12.
ATLAS,R.M. 1981. Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiological Reviews. 45(1):180-209.
GOPINATH, S.C.B., P. ANBU and A. HILDA. 2005. Extracellular enzymatic activity profiles in fungi isolated from oil rich environments. Mycoscience. 46:119-126.
HUSAINI, A, H.A ROSLAN and K.S.Y. HII. 2008. Biodegradation of alipathic hydrocarbon by indigenous fungi isolated from used motor oil contaminated sites. World Journal of Microbiology and Biotechnology 24:2789-2797.
LEAHY, J.G and R.R. COLWELL. 1990. Microbial degradation of hydrocarbons in the environment. Microbiological Reviews. 54(3):427-450.
LOTFINABASL, S., V.R. GUNALE and N.S. RAJURKAR. 2012. Assessment of petroleum hydrocarbon degradation from soil and tarball by fungi. Bioscience Discovery. 3(2):186-192.
MIRANDA, R.D.C., C.S. DE SOUZA, E.D.B. GOMES, R.B. LOVAGLIO, C.E. LOPES and M.D.F.V.D.Q. SOUSA. 2007. Biodegradation of diesel oil by yeasts isolated from the vicinity of Suape Port in the State of Pernambuco-Brazil. Brazilian Archives of Biology and Technology. 50(1):147-152.
OBIRE, O., E.C. ANYANWU, and R.N. OKIGBO. 2008. Saprophytic and crude oil degrading fungi from cow dung and poultry droppings as bioremediating agents. Journal of Agricultural Technology. 4(2):81-89.
ODGEN, R. and D.A.ADAMS. 1989. Recombinant DNA Technology: Applications. IN: Carolina Tips. Carolina Biological Supply Company, Burlington, North Carolina. 52:82-91.
OJUMU, T.V., O.O. BELLO, J.A. SONIBARE and B.O. SOLOMON. 2004. Evaluation of microbial systems for bioremediation of petroleum refinery effluents in Nigeria. African Journal of Biotechnology. 4(1):31-35.
OKAFOR, U.G., F. TASIE and F.M. OKAFOR. 2009. Hydrocarbon degradation potentials of indigenous fungal isolates from petroleum contaminated soils. Journal of Physical and Natural Sciences. 3(1):1-6.
PIMDA, W. and S. BUNNAG. 2012. Biodegradation of used motor oil by single and mixed cultures of cyanobacteria. African Journal of Biotechnology. 11(37):9074-9078.
SEPAHI, A.A., I.D. GOLPASHA, M. EMAMI and A.M.NAKHODA. 2008. Isolation and Characterization of Crude Oil Degrading Bacillus spp.. Iranian Journal of Environmental Health Science and Engineering 5(3):149-154.
TAGAYTAY, G.M.P. 2007. Isolation and identification of marine fungi in the, gills of Siganus fuscescens (Huttyn) from Hilapnitan, Baybay City, Leyte. Unpublished BS Thesis. Visayas State University, Visca, Baybay City, Leyte. pp 8-13.