Detection of Copper (II) and Iron (III) in Aqueous Solutions Using the Spectroscopic Characteristics of Bugnay (Antidesma bunius) Anthocyanins

Jonathan  M. Barcelo,; Jan Narlo  Abril,; Khristine Mereille  P. Castillo,; Alloisa  Diaz,; Jonathan Paul  Ladera,; Jaemie  Javar,; Elisha  Labuguen,

Detection of Copper (II) and Iron (III) in Aqueous Solutions Using the Spectroscopic Characteristics of Bugnay (Antidesma bunius) Anthocyanins

Abstract:

The spectrophotometric characteristics of bugnay (Antidesma bunius) anthocyanins in acidified solutions of copper (Cu ) and iron (Fe ) were investigated after one hour of reaction to determine the changes in their absorbance characteristics. Anthocyanins from bugnay were isolated using solid phase extraction followed by evaporation at 40 C. The total anthocyanin content of the extract was determined to be 103.87 ± 2.91 mg/L cyanidin-3- O-glucoside equivalents using pH differential method. Maximum absorbance readings at pH 1.0 and 4.5 were determined to be at 520 nm and 350 nm, respectively. Cyanidin-3-glucoside was identified as one of the components of the three pigments in the extract using reversed phase high performance liquid chromatography. At pH 1.0, copper caused greater hypochromic shift of bugnai anthocyanins compared to iron (ρ<0.01) while iron caused greater hypochromic shift at pH 4.5. Copper also caused hypsochromic shift of anthocyanins from 520 nm to 350 nm at pH 1.0 but not at pH 4.5. Correlation analysis showed a significant moderate positive correlation between mean % hypochromic shift and concentration of copper ions at pH 1.0 ( R2= 0.603, ρ<0.01) and 4.5 ( = 0.533, ρ<0.01), and iron at pH 4.5 (R2 = 0.638, ρ<0.01). The spectroscopic characteristics of bugnay anthocyanins at 350 nm and 520 nm can be used as parameters to detect copper and iron in acidic solutions.

References:

ACAR, O., OZVATAN, S. and ILIM, M. 2005. Determination of cadmium, copper, iron, manganese, lead and zinc in lichens and botanic samples by electrothermal and flame atomic absorption spectrometry. Turkish Journal of Chemistry 29, 335-344.
AHMED, MJ. And ROY, UK. A simple spectrophotometric method for the determination of iron (II) aqueous solutions. Turkish Journal of Chemistry 33,709-726.
AOAC OFFICIAL METHOD 2005.02. 2006. Total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines-pH differential method first action 2005. Journal of Association of Analytical Chemists International 88:1269.
AMELIA, F., AFNANI, G., MUSFIROH, A., FIKRIYANI, A., UCCHE, S., and MURRUKMIHADI, M. 2006. Extraction and stability test of anthocyanin from buni fruits (Antidesma Bunius L) as an alternative natural and safe food colorants. Journal of Food and Pharmacologic Sciences 1:49-53.
BROUILLARD, R., CHASSAING S., ISOREZ G., KUENY-STOTZ M., and FIGUEIREDO P. 2010. The visible flavonoids or anthocyanins: From research to applications. Recent Advances in Polyphenol Research 2:1-22.
CHENG, G.. and CRISOSTO, C. 1997. Iron-polyphenol complex formation and skin discoloration in peaches and nectarines. Journal of the American Society for Horticultural Science 122(1):95-99.
GEORGE, F., FIGUEIREDO, P., and BROUILLARD, R. 1999. Malvin Z-chalcone: An unexpected new open cavity for the ferric ion. Phytochemistry 50:1391-1394
JENSHIROOBHA, J., SARAVANAKUMAR, M., ARAVINDHAN, K., and SUGANYA DEVI, P. 2011. The effect of light, temperature, pH on stability of anthocyanin pigments in Musa acuminata bract. Research in Plant Biology 1(5):5-12.
LAVIE, R. 1998. Iron and copper overload. Consumer Health 21(6).
LI, H., GUO, A., and WANG, H. 2008. Mechanisms of oxidative browning of wine. Food Chemistry 108:1-13.
KONCZAK, I. and ZHANG, W. 2004. Anthocyanins-More than nature’s colours. Journal of Biomedicine and Biotechnology 5:239-240.
MONCADA, M., MOURA, S., MELO, J., ROQUE, A., LODEIRO, C., and PINA, F. 2003. Complexation of aluminum (III) by anthocyanins and synthetic flavylium salts; a source for blue and purple color. Inorganic Chemica Acta 356:51-61.
MORTON, J. 1987. Fruits of Warm Climates. Creative Resource Systems, Inc.: 210-212.
OZER, A., and TUMEN, F. 2005. Cu (II) adsorption from aqueous solutions on sugar beet pulp carbon. The European Journal of Mineral Processing and Environmental Protection 5(1):26-34.
PATRUDU, T.B. and RAJU, K.V. 2011. Reductimetric determination of copper (II) with iron (II) in phosphoric acid medium and in presence of bromide ion. Research Journal of Chemical Sciences 1(3): 6-9.
PYYSALO, H. and KUUSI, T. 1973. The role of iron and tin in discoloration of berry and red beet juices. Zeitschrift für Lebensmittel Untersuchung und Forschung 53:224-233.
QIN, C., LI, Y., NIU, W., DING, Y., ZHANG, R. and SHAN, X. 2010. Analysis and Characterisation of Anthocyanins in Mulberry Fruit. Czech Journal of Food Sciences 28(2): 117-126.
REIN, M. 2005. Copigmentation reactions and color stability of berry anthocyanins. Academic Dissertation of University of Helsinki, Department of Applied Chemistry and Microbiology.
SARMA, A and SHARMA, R. 1999. Anthocyanin-DNA copigmentation: Mutual protection against oxidative damage. Phytochemistry 52:1313-1318.
SARMA, A., SREELAKSHMI, Y. and SHARMA, R. 1997. Antioxidant ability of anthocyanins against ascorbic acid oxidation. Phytochemistry 45(4):671-674.
WROLSTAD, R., DURST, R. and LEE, J. 2005. Tracking color and pigment changes in anthocyanin products. Trends in Food Science and Technology 16:423-428.