Ontogenetic Differentiation Of Alpha And Beta Esterases In Univoltine, Bivoltine And Trimoulter Races Of Silkworm Bombyx Mori L.

Nidaghatta  B. Krishnamurthy,; P.  J. Raju,

Ontogenetic Differentiation Of Alpha And Beta Esterases In Univoltine, Bivoltine And Trimoulter Races Of Silkworm Bombyx Mori L.

P. J. Raju | Nidaghatta B. Krishnamurthy

Abstract:

Formation of an adult organism from a single fertilized cell is the result of selective differential gene expression, operating on a constant pool of genetic information. The differential gene action can be viewed by the differential synthesis and activity of certain enzymes. The variation patterns in two enzyme systems of alpha and beta esterases have been studied in a univoltine race I-380, bivoltine NB,D2 and in a trimoulter race of silkworm Bombyx mori L. In total, 10 different development stages of each of the races were analysed. The number of bands and their intensity varied during different developmental stages indicating differential gene action. The pattern of variation of enzyme bands and their significance are herein discussed.

References:

ABRAHAM, E.G., NAGARAJU, J. and DATTA, R.K. 1992. Biochemical studies of amylases in the silkworm, Bombyx mori L.: Comparative analysis in diapausing and non-diapausing strains. Insect Biochem. Molec. Biol. 22(8):863-873.
AYALA, F.J., POWELL, J.R., TRACEY, M.L., MOURAO, C.A. and PEREZSALAS, S. 1972. Enzyme variability in the Drosophila willistoni group. IV - Genic variation in natural populations of Drosophila willistoni. Genetics 70:113-139.
AYALA, F.J., TRACEY, M.L., BARR, L.G. and EHRENFELD, J.G. 1974. Genetic reproductive differentiation of the sub species, Drosophila equinoxialis carribiensis. Evolution 28:24-41.
DAVIS, B.J. 1964. Disc electrophoresis II: Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121:404-427.
GAMO, T. 1983. Bio-chemical genetics and its application for breeding silkworm. JARQ 16:264-272.
HEGDE, S.N. and KRISHNAMURTHY, N.B. 1980. Ontogenetic differentiation of alkaline and acid phosphatases in two races of silkworm Bombyx mori (L). The Ind. Zoologist 4(1&2):27-32.
JOHNSON, F.M., KANAPI, C.G., RICHARDSON, R.H., WHEELER, M.R. and STONE, W.S. 1966. An operational classification of Drosophila esterases for species comparisons. Studies in Genetics 6651:517-532.
ΚΑΙ, H., KAWAI, T. and KAWAI, Y. 1986. Purification of esterase A4 from Bombyx eggs. J. Seric. Sci. Jpn. 55(1):77-78.
KELLER, E.C. and GLASSMAN, E. 1964. Selection for Xanthine dehydrogenase differences in activity among Drosophila strains. Sci. 143:40-41.
MARKERT, C.L. 1963. Epigenetic control of specific protein synthesis in differentiating cells. In Cyto-differentiation and molecular synthesis. Locke, M. (Ed.) Academic Press, N.Y. pp. 68-84.
MASTERS, C.J. and HOLMES, R.S. 1972. Isozymes and ontogeny. Biol. Rev. 47-309-361
MOROSHIMA, I. 1975. Cyclic nucleotide phosphodiesterase in silkworm. Developmental change of cyclic AMP and cyclic GMP phosphodiesterase. Biochem. Biophisik. Acta. 403:106-112.
SUBRAMANYA, G. and REDDY, G.S. 1982. Genetic differences between the mutant with shorter larval duration and the standard in Pure Mysore race of silkworm Bombyx mori L. Sericologica 22(4):196-202.