In Vitro Propagation Of Citrus Maxima (Burm) Merr.

Darlyn  B. Posas,; Marilyn  M. Belarmino,

In Vitro Propagation Of Citrus Maxima (Burm) Merr.

Abstract:

A system for in vitro propagation of pummelo orCitrus maxima (Burm) Merr. was established using shoot-tip and single-node stem segment taken from young seedlings. The procedure involved the initiation of shoots from explant tissue, followed by shoot proliferation,in vitro rooting, and finally, hardening of plantlets and potting out in soil. The agar-solidified Murashige and Skoog (MS) medium containing 30 g/L sucrose and supplemented with 0.2 mg/L BAP, 0.5 mg/L IBA and 40 mg/L adenine (MSI medium) was optimum for shoot initiation from both explants. For rapid shoot proliferation, the MS medium containing 0.5 mg/L BAP, 0.5 mg/L IBA and 40 mg/L adenine (MSP medium) was optimum. Eight-week-old shoots efficiently produced roots in agar-solidified MS medium containing 0.5 to 1.0 mg/L IBA compared with 4-wk-old shoots, indicating the importance of an adequate shoot growth prior to root induction.Using this system, 126 transplantable plantlets and 494 6-wk-old shoots can be produced from one nodal explant in a 4-month micropropagation cycle. One shoot tip explant can produce 26 transplantable plantlets and 50 6-wk-old shoots after 4 months.

References:

BARLASS, M. andK.G.M. SKENE. 1982. In vitro plantlet formation from Citrus species and hybrids. Scientia Hortic. 17:333-341.
BHANSALI, R. R. and H.C. ARYA. 1982. Differentiation in explants of Citrus paradisi Macf. (grapefruit) grown in culture. Indian J. Exp. Biol. 16:409-411.
BELARMINO, M.M., T. ABE and T. SASAHARA 1992. Efficient plant regeneration from leaf calli of Ipomoea batatas (L.) Lam. and its related species. Japan. J Breed. 42: 109-114.
BELARMINO, M.M., T.ABE and T. SASAHARA. 1994. Plant regeneration from stem and petiole protoplasts of sweetpotato (Ipomoea batatas) and its wild relative, I. lacunosa. Japan. J. Breed. 42: 109-114.
DE VRIES, D.P. andL.A.M. DUBOIS 1988. The effect of BAP and IBA on sprouting and adventitious root formation of 'Amanda' rose single-node softwood cuttings. Scientia Hortic. 34: 115-121.
GARDNER, R.C. 1993. Citrus (Citrus species, Rutaceae, dicotyledon). Scientia Hortic. 55:67-68.
GEORGE, E.F. and S.D.SHERRINGTON. 1984. Plant Propagation by Tissue Culture: Handbook and Directory of Commercial Laboratories. Exegetics Ltd. 690 pp.
GROUT, B.W. 1975. Wax development on leaf surfaces of Brassica oleraceae var. Currawong regenerated from meristem culture. Plant Sci. Lett. 5: 401-405.
HASEGAWA, P.M. 1980. Factors affecting shoot and root initiation from cultured rose shoot tips. J. Am. Soc. Hortic. Sci. 105: 216-220,
HAISSIG, B.E. 1979. Influence of aryl esters of indole-3-acetic and indole-3-butyric acids on adventitious root primordium initiation and development. Physiol. Plant. 47: 29-33.
HU, Y. andJ. WANG. 1983. In vitro graft enhanced nucellar plant development in the monembryonic Citrus grandis L. J. Hortic. Sci. 63(4): 705-709
KITTO, S.L. and M.S. YOUNG. 1981. In vitro propagation of Carrizo citrange. Hortic. Sci. 14: 20.
MURASHIGE, T. 1987. Cloning plants by tissue culture: Early years, current status and future prospects. Acta Hortic. 212: 35-42.
MURASHIGE, T. and D.P.H.TUCKER 1969. Growth factor requirements of Citrus tissue culture. Proc. First Citrus Symp. 3: 1155-1161.
NAUER, E.M., C.N.ROISTACHER, T.L. CARSON and T. MURASHIGE. 1983. In vitro shoot tip grafting to eliminate citrus viruses and virus-like pathogens produces uniform budlines. Hortic. Sci. 18(3): 308-309.
NEL, M. 1987. In vitro culture of citrus meristems. Info. Bull. Citrus and Subtropical Fruit Res. Inst., South Africa 175: 9.
PIERRE, I. 1994. Thein vitro technique: Their contribution to breeding and multiplication of ornamentals. Acta Hortic. 653: 22-24.
SAUTON, A., A. MOURAS and A. LUTZ. 1982. Plant regeneration from Citrus root meristems. J. Hortic. Sci. 57: 227-231.
SIM, G.E., C.J.GOH and C.S. LOH. 1989. Micropropagation of Citrus mitis Blanco: Multiple bud formation from shoot and root explants in the presence of 6-benzylaminopurine. Plant Sci. 59: 203-210.
SINGH, S., B.K. RAY, S. BHATTACHARYYA and P.C. DEKA. 1994. In vitro propagation of Citrus reticulata Blanco and Citrus limon Burm.f. Hortic. Sci. 29(3): 214-216.
SPIEGEL-ROY, P. and A. VARDI. 1984. Citrus. In P. V. Ammirato, D.A. Evans, W.R. Sharp and Y. Yamada (Eds.). Handbook of Plant Cell Culture. Vol. 3, Macmillan, New York. pp. 355-372.