HomeAnnals of Tropical Researchvol. 8 no. 4 (1986)

Effects Of Ph On Glucoamylase Production By Aspergillus Awamori Nrrl 3112 In An Airlift Fermenter

Andresito D. Acabal | Ernesto J. del Rosario

 

Abstract:

The effects of pH on batch production of glucoamylase by Aspergillus awamori NRRL 3112 in a 3.5-L airlift fermenter were determined using a mixture of cassava flour and rice bran (1:2 weight ratio) as substrate. Highest production of the enzyme was noted on the fourth day of incubation when the pH of the medium was maintained at 5.5. The volumetric and specific activities of glucoamylase were 124.4 IU/mL and 10.3 IU/mg protein, respectively.



References:

  1. BACHLER, M.J., STRANBERG, G.W. and SMILEY, K.L. 1970. Starch conversion by immobilized glucoamylase. Biotechnol. Bioeng. 12:85-92.
  2. COLLE, B.D., GARCIA, J.C.L. and DEL ROSARIO, E.J. 1982. Evalu-ation of several yeast isolates and Zymomonas strains for alcohol pro-duction. Paper presented at the Philsutech Convention. August 4-6, 1982, Cebu City.
  3. DAVIES, R. 1963. Microbial extracellular enzymes, their uses and some factors affecting their formation. In Biochemistry of Industrial Micro-organisms. C. Rainbow and A.H. Rose (eds.). Academic Press, New York. 68 p.
  4. DWORZCHAK, R. and NELSON, C.A. 1972. Fermentative production of glucoamylase. Standard Brad Inc., U.S. Patent No. 3, 660, 236. Chem. Abstr. 77:32471.
  5. GOULD, B.J. 1975. Principles of industrial enzymology. In Handbook of Enzyme Biotechnology. A. Wiseman (ed.). Ellis Horwood Ltd., New York. 9 p.
  6. KIK, M.C. 1956. Nutrients in rice bran and rice polish and improvement of protein quality with amino acids. J. Agric. Food Chem. 4:170-172.
  7. LOWRY, O.H. ROSEBROUGH, N.J., FARR, A.L. and RANDALL, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265.
  8. MILLER, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31:426-428.
  9. NITHIANDAM, V.S., SRINIVSAN, K.S.V., THOMAS JOSEPH, K. and SANTAPPA, M. 1981. Preparation and properties of immobilized amyloglucosidase. Biotechnol. Bioeng. 23:2273-2282.
  10. OSORIO, M.E. 1981. Optimization of glucoamylase production in shake-flask culture of Aspergillus awamori NRRL 3112. Unpublished B.S. Thesis, UPLB, College, Laguna.
  11. SMILEY, K.L., CADMUS, M.C., HENSLEY, D.E. and LAGODA, A.A. 1964. High-potency amyloglucosidase producing mold of the Aspergillus niger group. Appl. Microbiol. 12:455.
  12. SMILEY, K.L., HENSLEY, D.E., SMILEY, M.J. and GASDOREF, H.I.J. 1971. Kinetic patterns of glucoamylase isozymes isolated from Aspergillus species. Arch. Biochem. Biophys. 144:694-699.