HomeIAMURE International Journal of Ecology and Conservationvol. 6 no. 1 (2013)

In vitro Tissue Culture of Broccoli (Brassica oleracea var. Italica Curd)

Alma B. Segismundo | May Evelia V. Ruadap

Discipline: Biology, Botany, Natural Sciences



This experimental research determined the feasibility of micropropagating in vitro, broccoli from its curd through plant tissue culture technology to be able to produce plants that are free from any disease. The growth of broccoli (Brassica oleracea var Italica curd in vitro was measured in terms of organ formation and the cultures’ average height (cm) after the observation period. It also determined the percent survivorship of the plant cultures after the observation period. The method used was adopted from Balcita (2011) utilizing the modified Murashige and Skoog (MS) medium. Result of the study show that broccoli plantlets grew from the curd after a six-week observation period. Addition of thiamine in the culture medium protected the cultures from bacterial or fungal infections, thus coming up with very healthy plantlets and a high survivorship of the cultures was also observed. In conclusion, propagation of broccoli curd in vitro is feasible using the modified Murashige and Skoog (MS) medium; plantlets with roots, stem, leaves, and curd with florets can grow directly from the broccoli curd; and there is a high percent survivorship in the cultures with the addition of thiamine in the culture medium.