Home⇛Science Asia Review⇛vol. 11 no. 1 (2025)

Antimicrobial Activity of SemiPurified Ethanolic Extracts of Sea Lettuce (Ulva lactuca) and Ribbon Sea Lettuce (Ulva reticulata) Against Selected Pathogenic Microbes

An-Najwa Muhaiminah M. Tawasil | Roxanne Marie F. Ebol | Cris Angelo G. Revillas | Ben-Frazier U. Sabtula

Discipline: microbiology and cell science

 

Abstract:

Antimicrobial Resistance (AMR) has been a significant challenge in healthcare. This research study is aimed at investigating the potential antimicrobial activity of Ulvaceae species, Sea Lettuce (Ulva lactuca) and Ribbon Sea Lettuce (Ulva reticulata), through sourcing out its secondary metabolites against specific pathogenic microbes, Escherichia coli (gramnegative bacteria) and Staphylococcus aureus(gram-positive bacteria). The study employed an Experimental Research Design, which yielded results that have shown Treatment A (U. lactuca Semi-Purified Ethanolic Extract), specifically A1 (100%), with a mean recorded 7.33, to exhibit a potential gram-positive inhibitory effect. Meanwhile, Treatment B (U. reticulata Semi-Purified Ethanolic Extract), specifically B1 (100%), with a mean of 6.33, has a potential inhibition against gram-negative bacteria. ANOVA revealed that Treatment A and Treatment B have shown a p-value of 0.000, which is lower than the assigned p-value of 0.05. Hence, there is a notable difference between their antimicrobial activity in terms of the diameters of their zones of inhibition against Staphylococcus aureus and Escherichia coli when compared with the positive control (Ampicillin). Thus, Ulva lactuca and Ulva reticulata Semi-Purified Ethanolic Extracts have potential antimicrobial activity against specific pathogenic microbes. The experimentation, testing, data collection, and other materials such as the positive control (Ampicillin), as well as the bacterial preparation of E. coli and S. aureus (prepared through bacterial culture), were all conducted at the Universidad de Zamboanga (UZ) Laboratory Building, located at Don Toribio St., Tetuan, Zamboanga City. Meanwhile, the experimental variables, Ulva lactuca and Ulva reticulata, were collected on the shore of Maasin, Zamboanga City.



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