Effects Of Ethyl Methane Sulfonate And Co60 Gamma Irradiation In Winged Bean

Fernando  A. Bernardo,; Jejoma  G. Armachuelo,

Effects Of Ethyl Methane Sulfonate And Co60 Gamma Irradiation In Winged Bean

Jejoma G. Armachuelo | Fernando A. Bernardo

Abstract:

Mutation and other effects of Co60 gamma radiation and ethyl methane sulfonate (EMS) in winged bean were determined at the M1 generation. Seed germination was significantly reduced by irradiation at all levels used but only at 0.15% and above for EMS. Delayed seedling emergence, high lethality and pronounced morphological and probably physiological damage due to chromosomal aberrations resulted with the radiation treatments but not with EMS at the doses used. Chlorophyll mutations were observed as yellowish emergent seedlings resulting from EMS treatments and yellow variegations and leaf streaking associated with leaf deformations with irradiation. Violet flowers and seeds were also observed in both mutagen treatments but could not be ascertained as true mutations since the seeds used were not tested for purity. On the yield components, significant reduction in number of seeds per pod, and yields of pods, seeds and tubers were observed in the radiation treatments. Doses of 15 and 25 kr, however, resulted in a higher tuber yield compared with the control signifying a likely induction of favorable gene mutations. No significant effects were induced by EMS treatments on any yield parameter.

References:

BROCK, R.D., SHAW, H.F. and CALLEN, D.F. 1972. Induced variation in quantita-tively inherited character. Induced Mutations and Plant Improvement. Vienna: Inter-national Atomic Energy Commission. pp. 317-320.
CONGER, B.V., KONZAK, C.F. and NILAN, R.A. 1970. Methods of pre- and post-treatments. Manual on Mutation Breeding. pp. 48-52.
GAUL, H., FRIMMEL, G., GICHNER, T. and ULONSKA, E. 1972. Efficiency of mutagenesis. Induced Mutation and Plant Improvement. Vienna: International Atomic Energy Commission. pp. 121-139.
KHAN, T.N. and BROCK, R.D. 1975. Mutation breeding in winged bean. Regional Seminar on the use of Induced mutations in Plant Improvement of grain legume production in Southeast Asia. (Colombo, Sri Lanka, 8-12 Dec. 1975).
KONZAK, C.F., CONGER, B.V. and NILAN, R.A. 1970. Radiation sensitivity and modifying factors. Manual on Mutation Breeding. pp. 44-47.
KOO, F.K.S. 1972. Mutation breeding in soybeans. Induced Mutations and Plant Improvement. Vienna: International Atomic Energy Commission. pp. 285-292.
LUSE, R.A. 1970. Radiobiology. Manual on Mutation Breeding. International Atomic Energy Commission, Vienna: pp. 21-23.
МОН, С.С. 1972. Induced seed-coat color mutations in beans and their significance for bean improvement. Induced Mutations and Plant Improvement. Vienna: International Atomic Energy Commission. pp. 67-71.
NATIONAL ACADEMY OF SCIENCE. 1975. Winged bean, a high protein crop for the tropics. Washington, D.C. 2nd Printing.
NILAN, R.A., KLEINHOFS, A. and SIDERIS, E.G. 1969. Structural and biochemical concepts of mutations in flowering plants. Induced Mutations in Plant. Proceedings of a symposium, Pullman, 14-18, July 1969. Vienna: International Atomic Energy Commission. pp. 35-47.
SANTOS, I.S. 1969. Induction of mutations in mungbean (Phaseolus aureus ROXB) and genetic studies of some of the mutants. Induced Mutations in Plants. Proceedings of a symposium, Pullman, 14-18, July 1969. Vienna: International Atomic Energy Commission.
SENANAYAKE, Y.D.A. 1978. Radiosensitivity of seeds, duration of fertility and chemical characterization of winged bean, Psophocarpus tetragonolobus (L) DC, Workshop Seminar on the Development of the Potential of the Winged Bean, Los Baños, Laguna, Phil.
SMITH, H.H. 1972. Comparative genetic effects of different physical mutagens in higher plants. Induced Mutations and Plant Improvement. Vienna: International Atomic Energy Commission. pp. 75-92.