Determination Of Palmitic Acid Uptake By The Fat Body Of The Madera Roach

Nelson  M. Esguerra,; Eduardo  A. Macion,; Franklin  Chang,

Determination Of Palmitic Acid Uptake By The Fat Body Of The Madera Roach

Franklin Chang | Nelson M. Esguerra | Eduardo A. Macion

Abstract:

Uptake of [1.14C]palmitic acid by the fat body of female adult Leucophaea maderae was measured in vitro. The fat body free fatty acid fraction showed the highest radioactivity, followed by triglyceride. The other lipid fractions showed low incorporation of label. The turnover of palmitate to triglyceride in the fat body of L. maderae appears to be slower than those reported for other insect species. A method of pre-labelling the fat body with a radioisotope was found satisfactory and reliable.

References:

BEENAKKERS, A.M.T. 1965. Transport of fatty acids in Locusta migratoria during sus-tained flight. J. Insect Physiol. 11:879-888.
CHANG, F. 1971. A developmental analysis of the uptake and release of lipids by the fat-body of the tobacco hornworm, Manduca sexta. Insect Biochem. 1: 63-80.
CHANG, F. 1977. The presence of lipoprotein lipase activity and its relationship to lipid transport in the oleander hawkmoth, Deilephila nerii. Comp. Biochem. Physio. 57B: 209-214.
CHEESEMAN, P., GOLDSWORTHY, G.J. and MORDUE, W. 1977. Studies on the purification of locust adipokinetic hormone. Life Sciences 21: 231-236.
CHINO, H. and GILBERT, L.I. 1965. Lipid release and transport in insects. Biochem. Biophys. Acta. 98: 94-110.
CHIPPENDALE, G.M. 1971. Fat body and hemolymph lipids of the southwestern corn borer, Diatraea grandiosella, during metamorphosis. Insect Biochem. 1: 39-46.
COOK. B.J. and EDDINGTON, L.C. 1967. The release of triglycerides and free fatty acids from the fat body of the cockroach, Periplaneta americana. J. Insect Physiol. 13: 1361-1372.
FREEMAN, C.P. and WEST, D. 1966. Complete separation of lipid classes on a simple thin layer plate. J. Lipid Res. 1:324-328.
GILBERT, L.I., CHINO, H. and DOMROESE, K.J. 1965. Lipolytic activity of insect tissues and its significance in lipid transport. J. Insect Physiol. 11: 1057-1070.
GILBERT, L.I. and CHINO, H. 1974. Transport of lipids in insects. J. Lipid Res. 15: 439-456.
MAYER, R.J. and CANDY, D.J. 1969. Control of hemolymph lipid concentration during locust flight: Adipokinetic hormone from the corpora cardieca. J. Insect Physiol. 15: 611-620.
MARTIN, J.S. 1969. Studies on assimilation, mobilization, and transport of lipids by the fat body and hemolymph of Pyrrhocoris apterus. J. Insect Physiol. 15: 2319-2344.
ROBINSON, N.L. and GOLDSWORTHY, G.J. 1974. The effects of locust adipokinetic hormone on flight muscle metabolism in vivo and in vitro. J. Comp. Physiol. 89: 369-377.
STEVENSON, E. 1969. Monoglyceride lipase in moth flight muscle. J. Insect Physiol. 15: 1537-1550.
TIETZ, A. 1967. Fat transport in the locust: The role of diglycerides. Eur. J. Biochem. 2: 236-242.
WEIS-FOGH, T. 1952. Fat combustion and metabolic rate of flying locusts (Shistocerca gregaria Forskal). Phil. Trans. R. Soc. Ser. B. 237: 1-36.
WLODAWER, D., LAGWINSKA, E. and BARANSKA, J. 1966. Esterification of fatty acids in the wax moth hemolymph and its possible role in lipid transport. J. Insect Physiol. 12:547-560.